Fluorescence spectroscopy studies on 5-aminosalicylic acid and zinc 5-aminosalylicylate interaction with human serum albumin

The interaction between 5-aminosalicylic acid (5-ASA) or zinc 5-aminosalylicylate (5-ASZ) and human serum albumin (HSA) was studied by fluorescence spectroscopic technique. The binding constants of 5-ASA or 5-ASZ with HSA were determined at different temperatures under the optimum conditions. The bi...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2004-01, Vol.34 (1), p.189-197
Main Authors: Cui, Feng-Ling, Fan, Jing, Li, Wei, Fan, Yun-Chang, Hu, Zhi-De
Format: Article
Language:English
Subjects:
5-Aminosalicylic acid
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fluorescence spectroscopy
Fundamental and applied biological sciences. Psychology
General pharmacology
Human serum albumin
Humans
Medical sciences
Mesalamine - blood
Mesalamine - chemistry
Pharmacology. Drug treatments
Protein Binding - physiology
Serum Albumin - analysis
Spectrometry, Fluorescence - methods
Zinc - blood
Zinc - chemistry
Zinc 5-aminosalylicylate
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Summary:The interaction between 5-aminosalicylic acid (5-ASA) or zinc 5-aminosalylicylate (5-ASZ) and human serum albumin (HSA) was studied by fluorescence spectroscopic technique. The binding constants of 5-ASA or 5-ASZ with HSA were determined at different temperatures under the optimum conditions. The binding sites were obtained and the acting force were suggested to be mainly hydrophobic. The effect of common ions on the binding constants was also investigated. A fluorescence spectroscopy assay of the proteins is presented in the paper. The determination results of the proteins in human serum by this method are very close to those obtained using Coomassie Brilliant Blue G-250 colorimetry, with relative standard deviations of 0.8–2.9%. A practical method was proposed for the determination of 5-ASA or 5-ASZ in human serum samples.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.japna.2003.08.012