Time-course study of SCG10 mRNA levels associated with LTP induction and maintenance in the rat Schaffer-CA1 pathway in vivo

The maintenance of long-term potentiation (LTP) depends on altered gene expression. Previously, we found the expression of neuronal growth associated protein SCG10, which is involved in neurite outgrowth and neural regeneration, was up-regulated by LTP induction in the rat hippocampal Schaffer-colla...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 2004-01, Vol.120 (2), p.182-187
Main Authors: Peng, Haixiang, Derrick, Brian E., Martinez, Joe L.
Format: Article
Language:English
Subjects:
Animals
Autoradiography - methods
Biological and medical sciences
Carrier Proteins
Electric Stimulation
Excitatory Amino Acid Antagonists - pharmacology
Functional Laterality
Fundamental and applied biological sciences. Psychology
Hippocampus
Hippocampus - cytology
In Situ Hybridization - methods
Long-Term Potentiation - physiology
Long-Term Potentiation - radiation effects
Male
Membrane Proteins
Nerve Growth Factors - metabolism
Neural growth-associated protein
Neural Pathways - physiology
Neurons - metabolism
Neurons - physiology
NMDA receptor
Piperazines - pharmacology
Rats
Rats, Inbred F344
Real-time PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - metabolism
Schaffer-collateral pathway
Temporal
Time Factors
Vertebrates: nervous system and sense organs
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Summary:The maintenance of long-term potentiation (LTP) depends on altered gene expression. Previously, we found the expression of neuronal growth associated protein SCG10, which is involved in neurite outgrowth and neural regeneration, was up-regulated by LTP induction in the rat hippocampal Schaffer-collateral CA1 pathway. Here we studied the temporal expression pattern of SCG10 mRNA after LTP induction using permanently implanted electrodes in the same CA1 pathway. The real-time RT-PCR showed that both SCG10 mRNA 1 and 2 kb forms were increased at the 3 h, but not at 1 or 24 h. In situ hybridization revealed an increase of SCG10 2 kb mRNA level in ipsilateral CA3 and CA1 areas, but not their contralateral counterparts or either side of dentate gyrus. These results suggest that SCG10 may play a role in the maintenance of synaptic plasticity through a transient regulation of microtubule dynamics, which facilitates the structural remodeling of the presynaptic element during the consolidation period.
ISSN:0169-328X
1872-6941
DOI:10.1016/j.molbrainres.2003.10.009