Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1

We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exh...

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Bibliographic Details
Published in:Cancer letters 2004-02, Vol.204 (1), p.105-113
Main Authors: Kawai, Akira, Naito, Noriko, Yoshida, Aki, Morimoto, Yuki, Ouchida, Mamoru, Shimizu, Kenji, Beppu, Yasuo
Format: Article
Language:English
Subjects:
Adult
Animals
Biomarkers, Tumor - metabolism
Biphasic
Cell Division
Cell line
Chromosome Mapping
Chromosomes, Human, Pair 18 - genetics
Epithelial differentiation
Female
Humans
Karyotyping
Kinases
Mice
Mice, Inbred BALB C
Mice, Nude
Muscle Neoplasms - metabolism
Muscle Neoplasms - pathology
Oncogene Proteins, Fusion - metabolism
Polyclonal antibodies
Proto-Oncogene Proteins c-bcl-2
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sarcoma, Synovial - metabolism
Sarcoma, Synovial - pathology
Smooth muscle
Synovial sarcoma
SYT-SSX
Translocation, Genetic
Tumor Cells, Cultured - metabolism
Tumor Cells, Cultured - pathology
Tumors
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Summary:We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2003.09.031