Detection of circulating 54 kDa antigen in sera of bovine calves experimentally infected with F. gigantica

The antibody response and circulating antigen levels in bovine calves, infected experimentally with Fasciola gigantica, were monitored using enzyme-linked immunoelectrotransfer blot (EITB) and sandwich ELISA, respectively. By EITB, the infected calves’ sera recognized the polypeptides in the range o...

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Bibliographic Details
Published in:Veterinary parasitology 2004-01, Vol.119 (2), p.187-195
Main Authors: Velusamy, R., Singh, B.P., Sharma, R.L., Chandra, D.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - blood
Antigens, Helminth - blood
Blotting, Western - veterinary
Cattle
Cattle Diseases - blood
Cattle Diseases - immunology
Cattle Diseases - parasitology
Cattle-Trematoda
Circulating antigen
Diagnosis-Trematoda
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Fasciola - growth & development
Fasciola - immunology
Fasciola gigantica
Fascioliasis - immunology
Fascioliasis - parasitology
Fascioliasis - veterinary
Feces - parasitology
Male
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Summary:The antibody response and circulating antigen levels in bovine calves, infected experimentally with Fasciola gigantica, were monitored using enzyme-linked immunoelectrotransfer blot (EITB) and sandwich ELISA, respectively. By EITB, the infected calves’ sera recognized the polypeptides in the range of 54–58 kDa as early as 2 weeks post-infection. By 12th week post-infection, the lower two polypeptides of 12 and 8 kDa had disappeared. In sandwich ELISA, the circulating 54 kDa and whole worm antigen of F. gigantica were detected in the sera samples of infected calves as early as 2 weeks post-infection and persisted until the end of experiment (26th week PI). The 54 kDa antigen of F. gigantica appears to be specific and possesses promising immunodiagnostic potential for early prepatent diagnosis of bovine fasciolosis.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2003.09.023