Identification of the mouse neurochondrin promoter region and the responsible region for cell type specific gene regulation

• Published in: Neuroscience letters 2004-02, Vol.356 (2), p.107-110
• Main Authors: Dateki, Minori, Mochizuki, Reiko, Yanai, Kazuyuki, Fukamizu, Akiyoshi
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Activating region; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Cell type dependent effect; Cells, Cultured; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Erythroid-Specific DNA-Binding Factors; Fundamental and applied biological sciences. Psychology; GATA family transcriptional factor; GATA2 Transcription Factor; Gene Expression Regulation; Mice; Molecular Sequence Data; Nerve Tissue Proteins - genetics; Neurochondrin; Osteoblasts - physiology; Promoter; Promoter Regions, Genetic - genetics; Repressing region; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic - genetics; Transfection; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2003.11.026

Neurochondrin is a cytoplasmic protein possibly involved in neurite outgrowth and chondrocyte differentiation. In the present study, we have identified 202 bp of the mouse neurochondrin minimal promoter sequences encompassing the transcriptional initiation site, and both of the activating and repressing regions in the first exon. These two regulatory regions in the first exon had a cell type dependent effect on the identified minimal promoter. In the regulatory region, the duplication of potential binding sites for GATA family transcriptional factors was observed. Prospective binding sites for sex determining region Y and c-Ets1 were also found in the minimal promoter region. These factors could be potential regulators for the mouse neurochondrin gene.