Database on monoclonal antibodies to Cytokeratins

Cytokeratins (CK) are being extensively used as diagnostic markers for various malignancies and other diseases, including human oral precancer and cancer, due to their tissue specific expression. CK are epithelia specific intermediate filament (IF) proteins, which are expressed in a differentiation...

Full description

Saved in:
Bibliographic Details
Published in:Oral Oncology 2004-03, Vol.40 (3), p.236-256
Main Authors: Upasani, Ojaswini S, Vaidya, Milind M, Bhisey, Avinash N
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - classification
Antibodies, Monoclonal - immunology
Biological and medical sciences
Biomarkers, Tumor - analysis
Biomarkers, Tumor - immunology
Cytokeratins
Databases, Factual
Epitopes - immunology
Humans
Immunoblotting
Immunohistochemistry
Immunohistochemisty
Keratins - analysis
Keratins - immunology
Medical sciences
Monoclonal antibodies
Mouth Neoplasms - diagnosis
Otorhinolaryngology. Stomatology
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cytokeratins (CK) are being extensively used as diagnostic markers for various malignancies and other diseases, including human oral precancer and cancer, due to their tissue specific expression. CK are epithelia specific intermediate filament (IF) proteins, which are expressed in a differentiation dependent and tissue specific manner. There are about 30 polypeptides of CK expressed by different human epithelia. Each type of epithelium expresses about 4–6 polypeptides. CK polypeptides share many common epitopes, due to which the antibodies developed against CK tend to cross react. Therefore, a large number of monoclonal and polyclonal antibodies have been developed to distinguish among these proteins. Many of these antibodies are not only monospecific but are also epitope specific. These antibodies are being used in pathology laboratories for routine diagnosis using immunohistochemistry. A number of fixatives are used for fixation of tissue sections prior to the use of these antibodies. Sometimes, this leads in epitope masking. Hence, it becomes necessary to use a battery of monoclonal antibodies (MAb) for accurate diagnosis. Apart from the use of these antibodies in diagnostics, they are also being used in basic research for the study of CK function and their interactions with associated proteins and membrane proteins. In the present communication an effort has been made to make a comprehensive list of MAb to CK giving information like cross-reactivity, epitope specificity, various fixatives used, etc. along with the source of the antibodies, which will provide useful information to the users.
ISSN:1368-8375
1879-0593
DOI:10.1016/j.oraloncology.2003.08.022