Direct spectrophotometric determination of quercetin in the presence of ascorbic acid

The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medi...

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Bibliographic Details
Published in:Farmaco (Società chimica italiana : 1989) 2004, Vol.59 (1), p.21-24
Main Authors: Pejic, N, Kuntic, V, Vujic, Z, Micic, S
Format: Article
Language:English
Subjects:
Ascorbic Acid - chemistry
Hydrogen-Ion Concentration
Osmolar Concentration
Quercetin
Quercetin - analysis
Quercetin - chemistry
Solvents - chemistry
Spectrophotometric method
Spectrophotometry - methods
Temperature
Vitamin C
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Summary:The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34 °C and I = 7.5 × 10 –5 M. Beer’s law is obeyed in the concentration range 1.0–12.0 μg ml –1 for quercetin. The corresponding detection limit is 0.76 μg ml –1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab ®.
ISSN:0014-827X
1879-0569
DOI:10.1016/j.farmac.2003.07.013