Occurrence and structure of lipoteichoic acids in the genus Staphylococcus

Lipoteichoic acids were isolated from eleven species of the genus Staphylococcus using phenol-water partition and hydrophobic chromatography on octyl-Sepharose CL-4B. The lipoteichoic acids purified could be visualized by SDS-PAGE. They were shown to be composed of a hydrophilic poly(glycerophosphat...

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Bibliographic Details
Published in:Archives of microbiology 1990-09, Vol.154 (4), p.375-379
Main Authors: Ruhland, G J, Fiedler, F
Format: Article
Language:English
Subjects:
Chromatography, Gas
Chromatography, Gel
Chromatography, Thin Layer
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Molecular Structure
Staphylococcus - analysis
Staphylococcus hominis
Teichoic Acids - chemistry
Teichoic Acids - isolation & purification
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Summary:Lipoteichoic acids were isolated from eleven species of the genus Staphylococcus using phenol-water partition and hydrophobic chromatography on octyl-Sepharose CL-4B. The lipoteichoic acids purified could be visualized by SDS-PAGE. They were shown to be composed of a hydrophilic poly(glycerophosphate) chain covalently linked to gentiobiosyldiacylglycerol, the common lipid anchor of these molecules. Glycerophosphate units of the hydrophilic chain were found to be partly substituted with ester-linked D-alanine, except in the case of S. cohnii. The lipoteichoic acids isolated from S. cohnii, S. hominis, S. saprophyticus and S. simulans contain alpha(1-2)-linked N-acetylglucosamine as an additional substituent of the poly(glycerophosphate) backbone.
ISSN:0302-8933
1432-072X
DOI:10.1007/bf00276534