In vitro mononuclear cell production of tumour necrosis factor-α and weight loss

• Published in: Diabetes research and clinical practice 2004-03, Vol.63 (3), p.179-184
• Main Authors: Morris, Alison M, Noakes, Manny, Clifton, Peter M
• Format: Article
• Language: English
• Subjects: Adipose Tissue; Adult; Blood Glucose - analysis; Body Composition; Body Constitution; Body Mass Index; Cholesterol, HDL - blood; Cholesterol, LDL - blood; Diabetes Mellitus - blood; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diet, Reducing; Female; Humans; Insulin - blood; Leukocytes, Mononuclear - metabolism; Male; Mononuclear cell; Obesity; Obesity - complications; Obesity - diet therapy; TNF-α; Triglycerides - blood; Tumor Necrosis Factor-alpha - biosynthesis; Type 2 diabetes; Weight loss; Weight Loss - physiology
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/j.diabres.2003.09.012

Objective: Elevated tumour necrosis factor-alpha (TNF-α) production in adipose tissue has been associated with obesity. We investigated whether mononuclear cell production of TNF-α decreased with weight loss in an obese population. Research methods and procedures: Seventeen obese patients with type 2 diabetes (BMI 32.5±0.9 kg/m 2) and 33 obese, non-diabetic controls (BMI 31.2±0.5 kg/m 2) underwent 12 weeks of 30% total energy restriction (6622±84 KJ per day). Every 4 weeks, weight and blood pressure were measured and fasting venous blood was analysed for lipid, glucose and insulin concentrations. At the beginning and end of energy restriction, mononuclear cells were isolated from whole blood and TNF-α production measured by ELSIA. Results: TNF-α production was not associated with the degree of adiposity but was higher in diabetic subjects ( P