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In vitro mononuclear cell production of tumour necrosis factor-α and weight loss
Objective: Elevated tumour necrosis factor-alpha (TNF-α) production in adipose tissue has been associated with obesity. We investigated whether mononuclear cell production of TNF-α decreased with weight loss in an obese population. Research methods and procedures: Seventeen obese patients with type...
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| Published in: | Diabetes research and clinical practice 2004-03, Vol.63 (3), p.179-184 |
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| creator | Morris, Alison M Noakes, Manny Clifton, Peter M |
| description | Objective: Elevated tumour necrosis factor-alpha (TNF-α) production in adipose tissue has been associated with obesity. We investigated whether mononuclear cell production of TNF-α decreased with weight loss in an obese population.
Research methods and procedures: Seventeen obese patients with type 2 diabetes (BMI 32.5±0.9
kg/m
2) and 33 obese, non-diabetic controls (BMI 31.2±0.5
kg/m
2) underwent 12 weeks of 30% total energy restriction (6622±84
KJ per day). Every 4 weeks, weight and blood pressure were measured and fasting venous blood was analysed for lipid, glucose and insulin concentrations. At the beginning and end of energy restriction, mononuclear cells were isolated from whole blood and TNF-α production measured by ELSIA.
Results: TNF-α production was not associated with the degree of adiposity but was higher in diabetic subjects (
P |
| doi_str_mv | 10.1016/j.diabres.2003.09.012 |
| format | article |
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Research methods and procedures: Seventeen obese patients with type 2 diabetes (BMI 32.5±0.9
kg/m
2) and 33 obese, non-diabetic controls (BMI 31.2±0.5
kg/m
2) underwent 12 weeks of 30% total energy restriction (6622±84
KJ per day). Every 4 weeks, weight and blood pressure were measured and fasting venous blood was analysed for lipid, glucose and insulin concentrations. At the beginning and end of energy restriction, mononuclear cells were isolated from whole blood and TNF-α production measured by ELSIA.
Results: TNF-α production was not associated with the degree of adiposity but was higher in diabetic subjects (
P<0.04). There was a reduction after energy restriction (281±43 to 182±30
pg/ml,
P<0.05) however the presence of type 2 diabetes did not influence the magnitude of this change. Plasma glucose and insulin levels decreased after weight loss in all subjects and weak correlations were found with TNF-α concentrations (
r=0.3,
P<0.05).
Conclusions: We conclude that maximal production of TNF-α from mononuclear cells decreases with energy restriction and is weakly associated with plasma glucose and insulin concentrations in obese patients.</description><identifier>ISSN: 0168-8227</identifier><identifier>EISSN: 1872-8227</identifier><identifier>DOI: 10.1016/j.diabres.2003.09.012</identifier><identifier>PMID: 14757289</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adipose Tissue ; Adult ; Blood Glucose - analysis ; Body Composition ; Body Constitution ; Body Mass Index ; Cholesterol, HDL - blood ; Cholesterol, LDL - blood ; Diabetes Mellitus - blood ; Diabetes Mellitus, Type 2 - blood ; Diabetes Mellitus, Type 2 - complications ; Diet, Reducing ; Female ; Humans ; Insulin - blood ; Leukocytes, Mononuclear - metabolism ; Male ; Mononuclear cell ; Obesity ; Obesity - complications ; Obesity - diet therapy ; TNF-α ; Triglycerides - blood ; Tumor Necrosis Factor-alpha - biosynthesis ; Type 2 diabetes ; Weight loss ; Weight Loss - physiology</subject><ispartof>Diabetes research and clinical practice, 2004-03, Vol.63 (3), p.179-184</ispartof><rights>2003 Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c276t-93320431d498a54382c4e5a4f625048d43af6ebf3c1dc9d4a30effdfa131732e3</citedby><cites>FETCH-LOGICAL-c276t-93320431d498a54382c4e5a4f625048d43af6ebf3c1dc9d4a30effdfa131732e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14757289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morris, Alison M</creatorcontrib><creatorcontrib>Noakes, Manny</creatorcontrib><creatorcontrib>Clifton, Peter M</creatorcontrib><title>In vitro mononuclear cell production of tumour necrosis factor-α and weight loss</title><title>Diabetes research and clinical practice</title><addtitle>Diabetes Res Clin Pract</addtitle><description>Objective: Elevated tumour necrosis factor-alpha (TNF-α) production in adipose tissue has been associated with obesity. We investigated whether mononuclear cell production of TNF-α decreased with weight loss in an obese population.
Research methods and procedures: Seventeen obese patients with type 2 diabetes (BMI 32.5±0.9
kg/m
2) and 33 obese, non-diabetic controls (BMI 31.2±0.5
kg/m
2) underwent 12 weeks of 30% total energy restriction (6622±84
KJ per day). Every 4 weeks, weight and blood pressure were measured and fasting venous blood was analysed for lipid, glucose and insulin concentrations. At the beginning and end of energy restriction, mononuclear cells were isolated from whole blood and TNF-α production measured by ELSIA.
Results: TNF-α production was not associated with the degree of adiposity but was higher in diabetic subjects (
P<0.04). There was a reduction after energy restriction (281±43 to 182±30
pg/ml,
P<0.05) however the presence of type 2 diabetes did not influence the magnitude of this change. Plasma glucose and insulin levels decreased after weight loss in all subjects and weak correlations were found with TNF-α concentrations (
r=0.3,
P<0.05).
Conclusions: We conclude that maximal production of TNF-α from mononuclear cells decreases with energy restriction and is weakly associated with plasma glucose and insulin concentrations in obese patients.</description><subject>Adipose Tissue</subject><subject>Adult</subject><subject>Blood Glucose - analysis</subject><subject>Body Composition</subject><subject>Body Constitution</subject><subject>Body Mass Index</subject><subject>Cholesterol, HDL - blood</subject><subject>Cholesterol, LDL - blood</subject><subject>Diabetes Mellitus - blood</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diet, Reducing</subject><subject>Female</subject><subject>Humans</subject><subject>Insulin - blood</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Mononuclear cell</subject><subject>Obesity</subject><subject>Obesity - complications</subject><subject>Obesity - diet therapy</subject><subject>TNF-α</subject><subject>Triglycerides - blood</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Type 2 diabetes</subject><subject>Weight loss</subject><subject>Weight Loss - physiology</subject><issn>0168-8227</issn><issn>1872-8227</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkEtu2zAQhomiQe24PUICrrqTMnxIolZBETQPwEBQIFkTNDlsaUiiQ0oJcqxepGeKHBvoMquZxTcz_3yEnDEoGbD6Ylu6YDYJc8kBRAltCYx_IkumGl4ozpvPZDlz6r1fkNOctwBQC1l9IQsmm6rhql2SX3cDfQ5jirSPQxwm26FJ1GLX0V2KbrJjiAONno5TH6dEB7Qp5pCpN3aMqfj3l5rB0RcMv_-MtIs5fyUn3nQZvx3rijxe_3y4ui3W9zd3Vz_WheVNPRatEBykYE62ylRSKG4lVkb6mlcglZPC-Bo3XljmbOukEYDeO2-YYI3gKFbk-2HvnPNpwjzqPuR9cDNgnLJWwARwBTNYHcB98pzQ610KvUmvmoHeu9RbfXSp9y41tHp2Oc-dHw9Mmx7d_6mjvBm4PAA4v_kcMOlsAw4WXUhoR-1i-ODEG-NOiUI</recordid><startdate>200403</startdate><enddate>200403</enddate><creator>Morris, Alison M</creator><creator>Noakes, Manny</creator><creator>Clifton, Peter M</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200403</creationdate><title>In vitro mononuclear cell production of tumour necrosis factor-α and weight loss</title><author>Morris, Alison M ; Noakes, Manny ; Clifton, Peter M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-93320431d498a54382c4e5a4f625048d43af6ebf3c1dc9d4a30effdfa131732e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adipose Tissue</topic><topic>Adult</topic><topic>Blood Glucose - analysis</topic><topic>Body Composition</topic><topic>Body Constitution</topic><topic>Body Mass Index</topic><topic>Cholesterol, HDL - blood</topic><topic>Cholesterol, LDL - blood</topic><topic>Diabetes Mellitus - blood</topic><topic>Diabetes Mellitus, Type 2 - blood</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diet, Reducing</topic><topic>Female</topic><topic>Humans</topic><topic>Insulin - blood</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Mononuclear cell</topic><topic>Obesity</topic><topic>Obesity - complications</topic><topic>Obesity - diet therapy</topic><topic>TNF-α</topic><topic>Triglycerides - blood</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Type 2 diabetes</topic><topic>Weight loss</topic><topic>Weight Loss - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morris, Alison M</creatorcontrib><creatorcontrib>Noakes, Manny</creatorcontrib><creatorcontrib>Clifton, Peter M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diabetes research and clinical practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morris, Alison M</au><au>Noakes, Manny</au><au>Clifton, Peter M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro mononuclear cell production of tumour necrosis factor-α and weight loss</atitle><jtitle>Diabetes research and clinical practice</jtitle><addtitle>Diabetes Res Clin Pract</addtitle><date>2004-03</date><risdate>2004</risdate><volume>63</volume><issue>3</issue><spage>179</spage><epage>184</epage><pages>179-184</pages><issn>0168-8227</issn><eissn>1872-8227</eissn><abstract>Objective: Elevated tumour necrosis factor-alpha (TNF-α) production in adipose tissue has been associated with obesity. We investigated whether mononuclear cell production of TNF-α decreased with weight loss in an obese population.
Research methods and procedures: Seventeen obese patients with type 2 diabetes (BMI 32.5±0.9
kg/m
2) and 33 obese, non-diabetic controls (BMI 31.2±0.5
kg/m
2) underwent 12 weeks of 30% total energy restriction (6622±84
KJ per day). Every 4 weeks, weight and blood pressure were measured and fasting venous blood was analysed for lipid, glucose and insulin concentrations. At the beginning and end of energy restriction, mononuclear cells were isolated from whole blood and TNF-α production measured by ELSIA.
Results: TNF-α production was not associated with the degree of adiposity but was higher in diabetic subjects (
P<0.04). There was a reduction after energy restriction (281±43 to 182±30
pg/ml,
P<0.05) however the presence of type 2 diabetes did not influence the magnitude of this change. Plasma glucose and insulin levels decreased after weight loss in all subjects and weak correlations were found with TNF-α concentrations (
r=0.3,
P<0.05).
Conclusions: We conclude that maximal production of TNF-α from mononuclear cells decreases with energy restriction and is weakly associated with plasma glucose and insulin concentrations in obese patients.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>14757289</pmid><doi>10.1016/j.diabres.2003.09.012</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-8227 |
| ispartof | Diabetes research and clinical practice, 2004-03, Vol.63 (3), p.179-184 |
| issn | 0168-8227 1872-8227 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Adipose Tissue Adult Blood Glucose - analysis Body Composition Body Constitution Body Mass Index Cholesterol, HDL - blood Cholesterol, LDL - blood Diabetes Mellitus - blood Diabetes Mellitus, Type 2 - blood Diabetes Mellitus, Type 2 - complications Diet, Reducing Female Humans Insulin - blood Leukocytes, Mononuclear - metabolism Male Mononuclear cell Obesity Obesity - complications Obesity - diet therapy TNF-α Triglycerides - blood Tumor Necrosis Factor-alpha - biosynthesis Type 2 diabetes Weight loss Weight Loss - physiology |
| title | In vitro mononuclear cell production of tumour necrosis factor-α and weight loss |
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