Expression of p120, Ki-67 and PCNA as proliferation biomarkers in imprint smears of prostate carcinoma and their prognostic value

• Published in: Cytopathology (Oxford) 2004-02, Vol.15 (1), p.25-31
• Main Authors: Bantis, A., Giannopoulos, A., Gonidi, M., Liossi, A., Aggelonidou, E., Petrakakou, E., Athanassiades, P., Athanassiadou, P.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomarkers, Tumor - analysis; Carcinoma - metabolism; Carcinoma - mortality; Carcinoma - pathology; Disease-Free Survival; Humans; immunocytochemistry; Immunohistochemistry; Ki-67; Ki-67 Antigen - biosynthesis; Male; Middle Aged; Neoplasm Staging; Nuclear Proteins - biosynthesis; p120; Prognosis; prognostic factors; proliferating cell nuclear antigen; Proliferating Cell Nuclear Antigen - biosynthesis; prostate carcinoma; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - mortality; Prostatic Neoplasms - pathology; tRNA Methyltransferases
• ISSN: 0956-5507; 1365-2303
• DOI: 10.1046/j.0956-5507.2003.00090.x

The cell proliferation markers p120, Ki‐67 and proliferating cell nuclear antigen (PCNA) recognize nuclear antigens. The expression of these proteins by immunostaining methods was reported to be of value in determining the prognosis of patients with malignant diseases. In this study, we evaluated the prognostic significance of the expression of nuclear antigens p120, PCNA and Ki‐67 in prostate cancer and compared the results with other prognostic factors. Imprint smear samples obtained from 70 patients immediately after radical prostatectomy for prostatic carcinoma were immunostained with monoclonal antibodies against p120, Ki‐67 and PCNA. The immunostaining results were correlated with Gleason score, tumour differentiation, stage and prostatic specific antigen (PSA) levels. Our findings demonstrate that p120, Ki‐67 and PCNA expression in prostatic carcinoma smears, correlated significantly with the degree of Gleason score (P