Galactonate determination in urine by stable isotope dilution gas chromatography–mass spectrometry

A stable isotope dilution assay was developed for the sensitive determination of d-galactonic acid. d-[U- 13 C 6 ]galactono-1,4-lactone was prepared as internal standard. Unlabelled and U- 13 C -labelled d-galactonic acid species were converted to the N-(1-butyl)galactonamide pentaacetate derivative...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-03, Vol.801 (2), p.249-255
Main Authors: Schadewaldt, Peter, Hammen, Hans-Werner, Stolpmann, Sara, Kamalanathan, Loganathan, Wendel, Udo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbon Isotopes
Child
Child, Preschool
Female
Fundamental and applied biological sciences. Psychology
Galactitol - urine
Galactonate
Galactose - urine
Galactosemias - urine
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Humans
Indicator Dilution Techniques
Lactones - urine
Male
Medical sciences
Pharmacology. Drug treatments
Quality Control
Sensitivity and Specificity
Sugar Acids - urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A stable isotope dilution assay was developed for the sensitive determination of d-galactonic acid. d-[U- 13 C 6 ]galactono-1,4-lactone was prepared as internal standard. Unlabelled and U- 13 C -labelled d-galactonic acid species were converted to the N-(1-butyl)galactonamide pentaacetate derivatives and assessed by gas chromatography–mass spectrometry (GC–MS). Positive chemical ionisation and monitoring of the [ MH-60] +-ions in the galactonate chromatographic peak at m/ z 402 and m/ z 408 were used for quantification. The procedure was applied to study the variability of d-galactonate excretion in healthy subjects and galactosemic patients and to monitor the d-galactonate– d-galactitol ratio in human urine.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2003.11.020