Actions of the anti-oestrogen agent clomiphene on outward K+ currents in rat ventricular myocytes

• Published in: Clinical and experimental pharmacology & physiology 2004-01, Vol.31 (1-2), p.86-95
• Main Authors: Borg, John J, Hancox, Jules C, Hogg, Dayle S, James, Andrew F, Kozlowski, Roland Z
• Format: Article
• Language: English
• Subjects: Algorithms; Animals; anti-oestrogens; background K+ current; clomiphene; Clomiphene - pharmacology; Electrophysiology; Endothelin-1 - metabolism; Endothelin-1 - pharmacology; Estrogen Antagonists - pharmacology; Fertility Agents, Female - pharmacology; Heart Ventricles - cytology; Heart Ventricles - drug effects; Heart Ventricles - metabolism; In Vitro Techniques; Male; Membrane Potentials - drug effects; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Patch-Clamp Techniques; Potassium - pharmacology; Potassium Channel Blockers; Potassium Channels - drug effects; Potassium Channels - metabolism; rat ventricular myocytes; Rats; Rats, Wistar; Receptors, Endothelin - drug effects; Receptors, Endothelin - metabolism; transient outward K+ current; triphenylethylenes
• ISSN: 0305-1870; 1440-1681
• DOI: 10.1111/j.1440-1681.2004.03956.x

Summary 1. The effects of clomiphene (CLM) on cardiac outward K+ current components from rat isolated ventricular myocytes were investigated using the whole‐cell patch‐clamp technique. Clomiphene (10 µmol/L) significantly inhibited both peak (Ipeak) and end‐pulse (Ilate) outward currents (elicited by a 500 msec voltage step from −40 to +50 mV in the presence of K+‐containing intracellular and extracellular solutions) by approximately 37% (n = 6; P