Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis

Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel ele...

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Bibliographic Details
Published in:Analytical biochemistry 2004-02, Vol.325 (2), p.255-259
Main Authors: Zhang, Xinfeng, Ma, Yue, Liu, Hong, de Sa, Paula F.G, Brown, Phyllis R, Dain, Joel A
Format: Article
Language:English
Subjects:
AGE
Capillary electrophoresis
Electrophoresis, Capillary - methods
Electrophoresis, Polyacrylamide Gel
Fibrinogen
Fibrinogen - metabolism
Glucosamine
Glucosamine - metabolism
Glycation End Products, Advanced - analysis
Glycation End Products, Advanced - metabolism
Human serum albumin
Humans
Maillard Reaction
MRP
Serum Albumin - metabolism
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
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Summary:Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2003.11.001