Dietary-induced changes in the fatty acid profile of rat pancreatic membranes are associated with modifications in acinar cell function and signalling

• Published in: British journal of nutrition 2004-02, Vol.91 (2), p.227-234
• Main Authors: Yago, Maria D., Diaz, Ricardo J., Ramirez, Rolando, Martinez, Maria A., Mañas, Mariano, Martinez-Victoria, Emilio
• Format: Article
• Language: English
• Subjects: Amylases - metabolism; Animals; Biological and medical sciences; Calcium - metabolism; Calcium signalling; Cell Membrane - drug effects; Cell Membrane - metabolism; Cholecystokinin; Diet; Dietary fat; Dietary Fats, Unsaturated - pharmacology; Dose-Response Relationship, Drug; Enzymes; Fatty acids; Fatty Acids - metabolism; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastrointestinal Agents - pharmacology; Investigations; Laboratory animals; Lipids; Magnesium - metabolism; Male; Membrane fatty acids; Membranes; Nutrition; Oils & fats; Olive Oil; Pancreas - cytology; Pancreas - drug effects; Pancreas - secretion; Pancreatic acini; Plant Oils - pharmacology; Rats; Rats, Wistar; Signal transduction; Signal Transduction - physiology; Sincalide - pharmacology; Sunflower Oil; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Weaning
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN20031044

The effects of dietary lipids on the fatty acid composition of rat pancreatic membranes and acinar cell function were investigated. Weaning rats were fed for 8 weeks on one of two diets which contained 100 g virgin olive oil (OO) or sunflowerseed oil (SO)/kg. Pancreatic plasma membranes were isolated and fatty acids determined. Amylase secretion and cytosolic concentrations of Ca2+ and Mg2+ were measured in pancreatic acini. Membrane fatty acids were profoundly affected by the diets; the rats fed OO had higher levels of 18:1n-9 (42·86 (sem 1·99) %) and total MUFA compared with the animals fed SO (25·37 (sem 1·11) %). Reciprocally, the SO diet resulted in greater levels of total and n-6 PUFA than the OO diet. The most striking effect was observed for 18:2n-6 (SO 17·88 (sem 1·32) %; OO 4·45 (sem 0·60) %), although the levels of 20:4n-6 were also different. The proportion of total saturated fatty acids was similar in both groups, and there was only a slight, not significant (P=0·098), effect on the unsaturation index. Compared with the OO group, acinar cells from the rats fed SO secreted more amylase at rest but less in response to cholecystokinin octapeptide, and this was paralleled by reduced Ca2+ responses to the secretagogue. The results confirm that rat pancreatic cell membranes are strongly influenced by the type of dietary fat consumed and this is accompanied by a modulation of the secretory activity of pancreatic acinar cells that involves, at least in part, Ca2+ signalling.