Cytoplasmic transfer in the mouse in conjunction with intracytoplasmic sperm injection

Cytoplasmic transfer (CT) from a donor to a recipient oocyte has been used clinically to facilitate human pregnancies. Data reported here describe the first characterization of CT coincident with intracytoplasmic sperm injection in the mouse system. Sibling oocytes were used to transfer 2, 4, or 6 p...

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Bibliographic Details
Published in:Reproductive biomedicine online 2004, Vol.8 (1), p.75-80
Main Authors: Nagai, Seiichiro, Kasai, Tsuyoshi, Hirata, Shuji, Hoshi, Kazuhiko, Yanagimachi, Ryuzo, Huang, Thomas
Format: Article
Language:English
Subjects:
Animals
Blastocyst - physiology
Cell Survival
Cytoplasm
cytoplasmic transfer
Embryonic and Fetal Development
Female
intracytoplasmic sperm injection
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
oocyte reconstruction
Oocytes - physiology
ooplasm
Sperm Head
Sperm Injections, Intracytoplasmic - adverse effects
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Summary:Cytoplasmic transfer (CT) from a donor to a recipient oocyte has been used clinically to facilitate human pregnancies. Data reported here describe the first characterization of CT coincident with intracytoplasmic sperm injection in the mouse system. Sibling oocytes were used to transfer 2, 4, or 6 pl of ooplasm to a recipient egg along with a sperm head using piezo-actuated injection. Survival and fertilization after CT were comparable to controls at 2 pl and 4 pl, but survival was significantly reduced with 6 pl volumes. Development to the blastocyst stage was also inversely related to CT volume, with some decline beginning with the 4 pl CT group. However, some blastocysts did develop in all of the groups. The results are in contrast with human eggs, which tolerate larger CT volumes. Results indicate that the mouse system can be used to characterize the transfer of exogenous materials concomitant with sperm injection, provided that the CT volume is not excessive.
ISSN:1472-6483
1472-6491
DOI:10.1016/S1472-6483(10)60500-7