Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells

The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase...

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Bibliographic Details
Published in:FEBS letters 2004-01, Vol.558 (1), p.63-68
Main Authors: Muratovska, Aleksandra, Eccles, Michael R
Format: Article
Language:English
Subjects:
Animals
Carrier Proteins - metabolism
Cell Membrane - metabolism
Cercopithecus aethiops
CHO Cells
COS Cells
Cricetinae
Cricetulus
Cytoplasm - metabolism
Disulfides - metabolism
dsRNA, double-stranded RNA
Fibroblasts - metabolism
Galanin
Gene Silencing
Gene Transfer Techniques
Genes, Reporter
GFP, green fluorescent protein
Green Fluorescent Proteins
Luciferases - metabolism
Luminescent Proteins - metabolism
Mice
MPP, membrane permeant peptide
Penetratin
Peptide Fragments - metabolism
Recombinant Fusion Proteins - metabolism
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Short interfering RNA
siRNA, short interfering RNA
Transfection
Transgenes
Transportan
Wasp Venoms
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Summary:The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein ( GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)01505-9