Enhanced Chemiluminescence as a Measure of Oxygen-Derived Free Radical Generation During Ischemia and Reperfusion

It has been suggested that oxygen-derived free radicals may contribute to the myocardial injury associated with ischemia and reperfusion. As the presence of enhanced free radical generation is a prerequisite for such damage, several techniques have been used to provide evidence of increased oxygen f...

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Bibliographic Details
Published in:Circulation research 1990-12, Vol.67 (6), p.1453-1461
Main Authors: Henry, Timothy D, Archer, Stephen L, Nelson, Daniel, Weir, E Kenneth, L. From, Arthur H
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiology. Vascular system
Coronary Circulation
Coronary heart disease
Free Radicals
Heart
In Vitro Techniques
Ischemia
Luminescent Measurements
Male
Medical sciences
Myocardial Reperfusion
Oxygen
Rats
Rats, Inbred Strains
Superoxide Dismutase - pharmacology
Time Factors
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Summary:It has been suggested that oxygen-derived free radicals may contribute to the myocardial injury associated with ischemia and reperfusion. As the presence of enhanced free radical generation is a prerequisite for such damage, several techniques have been used to provide evidence of increased oxygen free radical production during reperfusion; however, all such techniques have substantial limitations. In this study, we used enhanced chemiluminescence to evaluate oxygen free radical generation during ischemia and reperfusion in the isolated Langendorff-perfused rat heart. The chemiluminescent technique, which has high sensitivity and can monitor radical generation continuously, avoids some of the limitations of earlier methods. Chemiluminescence (expressed as counts per second) decreased from 219±11 at baseline to 142±9 during ischemia and markedly increased to a peak of 476±36 during the first 3–5 minutes of reperfusion. This was followed by a slow decline over 11–16 minutes to a steady-state level of 253±14 (each sequential change in chemiluminescence was highly significant; p
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.67.6.1453