Evaluation of Multiwavelength Culture Fluorescence for Monitoring the Aroma Compound 4-Hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone (HEMF) Production

• Published in: Biotechnology progress 2004, Vol.20 (1), p.361-367
• Main Authors: Hagedorn, A., Levadoux, W., Groleau, D., Tartakovsky, B.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Cell Count - methods; Cell Culture Techniques - methods; Cell Division; Computer Simulation; Ethanol - metabolism; Fundamental and applied biological sciences. Psychology; Furans - metabolism; Glucose - metabolism; Models, Biological; Online Systems; Regression (Psychology); Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Spectrometry, Fluorescence - methods
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp0300321

Fluorescence spectra of a 4‐hydroxy‐2(or 5)‐ethyl‐5(or 2)‐methyl‐3(2 H)‐furanone (HEMF) fermentation culture broth were combined with measurable process variables for off‐line and on‐line process monitoring. Culture broth fluorescence in UV and visible ranges was acquired by a fiber optic LCD array spectrometer. Process dynamics was followed on‐line using a fiber optic probe attached to an external recirculation loop of the bioreactor. Partial least squares and stepwise regression methods were used to correlate measurable process parameters with the components of the fluorescence spectra. Both methods provided adequate approximation of yeast density, HEMF, glucose, and ethanol concentrations from fluorescence spectra. HEMF production was observed during the oxido‐reductive growth phase when there was a lack of measurable oxygen in the culture broth and an excess of glucose. The addition of glucose resulted in the rapid production of HEMF and other metabolite intermediates such as ethanol, acetate, and glycerol.