Distribution of the NisI immunity protein and enhancement of nisin activity by the lipid-free NisI

Lactococcus lactis cells producing the antibacterial peptide nisin protect their own cytoplasmic membrane by specific immunity proteins, NisI and NisF/E/G. We show here that approximately half of the produced NisI escaped the lipid modification (LF-NisI=lipid-free NisI) and was secreted to the mediu...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-02, Vol.231 (1), p.85-90
Main Authors: Koponen, Olli, Takala, Timo M, Saarela, Ulla, Qiao, Mingqiang, Saris, Per E.J
Format: Article
Language:English
Subjects:
Bacterial Proteins - immunology
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lactococcus
Lactococcus lactis
Lactococcus lactis - genetics
Lactococcus lactis - immunology
Lactococcus lactis - metabolism
Lipoproteins - genetics
Lipoproteins - immunology
Lipoproteins - metabolism
Membrane Proteins - immunology
Membrane Proteins - metabolism
Microbial Sensitivity Tests
Microbiology
Miscellaneous
NisI
Nisin
Nisin - genetics
Nisin - immunology
Nisin - metabolism
Nisin enhancer
Nisin immunity
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Summary:Lactococcus lactis cells producing the antibacterial peptide nisin protect their own cytoplasmic membrane by specific immunity proteins, NisI and NisF/E/G. We show here that approximately half of the produced NisI escaped the lipid modification (LF-NisI=lipid-free NisI) and was secreted to the medium, and that LF-NisI had no affinity to cells of L. lactis. The molar ratio of NisI and nisin was determined to be approximately 1:10 on the cell surface and 1:50 in the culture supernatant. Purified LF-NisI was shown to enhance the activity of nisin against several tested indicator strains. The enhancement of nisin activity by LF-NisI was not observed with cells containing the NisFEG transport system.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00934-0