Diazoxide causes early activation of cardiac sarcolemmal KATP channels during metabolic inhibition by an indirect mechanism

We have used isolated myocytes to investigate the effects of diazoxide on sarcolemmal KATP channel (sarcoKATP) activity and action potential failure during metabolic inhibition, and the role of these channels in protection of functional recovery on reperfusion. Isolated adult rat ventricular myocyte...

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Bibliographic Details
Published in:Cardiovascular research 2004-02, Vol.61 (3), p.570-579
Main Authors: RODRIGO, Glenn C, DAVIES, Noel W, STANDEN, Nicholas B
Format: Article
Language:English
Subjects:
Action Potentials - drug effects
Animals
Antihypertensive Agents - pharmacology
Biological and medical sciences
Calcium - metabolism
Cardiology. Vascular system
Diazoxide - pharmacology
Magnesium - metabolism
Male
Medical sciences
Myocardial Contraction
Myocardial Reperfusion Injury - metabolism
Myocytes, Cardiac
Patch-Clamp Techniques
Potassium Channel Blockers - pharmacology
Potassium Channels - metabolism
Rats
Rats, Wistar
Sarcolemma - drug effects
Sarcolemma - metabolism
Sulfonamides - pharmacology
Thiourea - analogs & derivatives
Thiourea - pharmacology
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Summary:We have used isolated myocytes to investigate the effects of diazoxide on sarcolemmal KATP channel (sarcoKATP) activity and action potential failure during metabolic inhibition, and the role of these channels in protection of functional recovery on reperfusion. Isolated adult rat ventricular myocytes were exposed to metabolic inhibition (NaCN and iodoacetate) and reperfusion. Functional recovery was assessed from the ability of cells to contract on electrical stimulation and to recover calcium homeostasis, measured with fura-2. Action potentials and KATP currents were measured using patch clamp. Pretreatment with diazoxide (100 microM, 5 min) increased the proportion of cells that recovered contractile function after MI and reperfusion from 16.8 +/- 2.4% to 65.0 +/- 2.2% (p
ISSN:0008-6363
1755-3245
DOI:10.1016/j.cardiores.2003.10.004