Evaluation of a RAPD‐based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil

Aims:  To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates. Materials and Results:  Seventy‐nine strains were examined by PCR for the toxin genes (ctx A, zot...

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Bibliographic Details
Published in:Journal of applied microbiology 2004-03, Vol.96 (3), p.447-454
Main Authors: Leal, N.C., Sobreira, M., Leal‐Balbino, T.C., Almeida, A.M.P., Silva, M.J.B., Mello, D.M., Seki, L.M., Hofer, E.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Brazil
Brazil - epidemiology
Cholera - epidemiology
Cholera - microbiology
Databases, Genetic
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
molecular epidemiology
Public Health Practice
Random Amplified Polymorphic DNA Technique
RAPD
Vibrio cholerae
Vibrio cholerae O1
Vibrio cholerae O1 - genetics
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Summary:Aims:  To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates. Materials and Results:  Seventy‐nine strains were examined by PCR for the toxin genes (ctx A, zot and ace), virulence‐associated genes (tcp A and tox T) and RAPD sequences. Except for one strain (no. 1123) from the Amazonas State, all the strains analysed carried the genes ctx A, zot, ace, tcp A and tox T. RAPD fingerprinting revealed variability but no correlation with serotype, biotype or geographical origin of the isolates was found. Conclusion:  A standardized RAPD method does not enable the establishment of a pattern data bank for the identification of V. cholerae O1 strains. Significance and Impact of the Study:  The simplicity and discriminative capacity of this technique make it useful for detecting genetic diversity among micro‐organisms from a defined group or for outbreak investigation.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2004.02090.x