A water-stable protected isocyanate glass array substrate

We describe the performance of a new glass attachment chemistry for arrays that is particularly well suited to attachment of small molecules, such as peptides. The attachment chemistry is a protected isocyanate (PI) group. Isocyanate groups are well suited to serving as a glass coating for arrays, i...

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Bibliographic Details
Published in:Analytical biochemistry 2004-03, Vol.326 (1), p.55-68
Main Authors: Sompuram, Seshi R, Vani, Kodela, Wei, Lan, Ramanathan, Halasya, Olken, Sarah, Bogen, Steven A
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Carbohydrates - analysis
Carbohydrates - chemistry
Diagnostics
DNA - analysis
DNA - chemistry
Drug Stability
Glass
Glass slide coating
Isocyanate
Isocyanates - chemistry
Kinetics
lipooligosaccharides
Mice
Microarrays
Molecular Probe Techniques - instrumentation
Peptides - analysis
Peptides - chemistry
Sensitivity and Specificity
Water - chemistry
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Summary:We describe the performance of a new glass attachment chemistry for arrays that is particularly well suited to attachment of small molecules, such as peptides. The attachment chemistry is a protected isocyanate (PI) group. Isocyanate groups are well suited to serving as a glass coating for arrays, in that they are highly reactive with many different types of biological compounds. However, they are generally so reactive as to be unstable. The new feature of the PI slide coating is its stability. It can withstand immersion in water without loss of reactivity and has at least a 1-year shelf life. The high reactivity of the PI group results in a rapid coupling reaction (
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2003.11.008