Enhanced expression of ganglioside GD3 in human and rat hepatocellular carcinoma cells and NIH 3T3 cells transfected with human tumor DNAs

The gangliosides of human hepatoma biopsies, human hepatoma cell lines, and diethylnitrosamine-induced rat hepatomas were examined. These malignant tissues all expressed increased content of disialolactosylceramide (GD3) with respect to their normal counterparts. During the induction of rat hepatoma...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1990-12, Vol.50 (23), p.7697-7702
Main Authors: JIAN NAN YE, TIAN-GUE GU, LIANG XIA, MURPHY, M. J, LEE, W, NENG-HAU GAO, JIAN-REN GU, FUHRER, J. P
Format: Article
Language:English
Subjects:
Animals
Antigens, Differentiation, T-Lymphocyte - biosynthesis
Biological and medical sciences
Carcinoma, Hepatocellular - metabolism
CD57 Antigens
Cell Line
Chromatography, Thin Layer
Diethylnitrosamine - pharmacology
Gangliosides - analysis
Gangliosides - biosynthesis
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation
Genes, ras
Humans
Hydrogen-Ion Concentration
Liver Neoplasms - metabolism
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Rats
Transfection
Tumor Cells, Cultured
Tumors
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Summary:The gangliosides of human hepatoma biopsies, human hepatoma cell lines, and diethylnitrosamine-induced rat hepatomas were examined. These malignant tissues all expressed increased content of disialolactosylceramide (GD3) with respect to their normal counterparts. During the induction of rat hepatoma by diethylnitrosamine, an increase in GD3 levels appeared as early as 12 wk after initiation of diethylnitrosamine, concurrent with the appearance of precancerous hepatocytes. GD3 levels gradually increased to a peak of 4 times that of normal rat liver at 20 wk. CMP-NeuAc:GM3 sialyltransferase, the enzyme that synthesizes GD3 by transfer of sialic acid to GM3, also had tumor-associated elevation during the course of diethylnitrosamine-induction of rat hepatomas. To investigate the relationship of oncogene transformation and changes in ganglioside biosynthesis, NIH 3T3 cells transfected DNAs from human hepatoma or nasopharyngeal carcinoma were studied. The transfectants each expressed the same ganglioside composition, including a detectable level of GD3, as well as enhanced activity of CMP-NeuAc:GM3 sialyltransferase. A correlation between the tumor DNA transfection and the augmentation of GD3 in malignant cells is discussed. Because of the early appearance of GD3 in hepatoma and its possible relationship to oncogene activation, GD3 may be a potentially useful early tumor marker.
ISSN:0008-5472
1538-7445