Production of lipocortin-like proteins by cultured human tracheal submucosal gland cells

• Published in: FEBS letters 1990-11, Vol.274 (1), p.131-135
• Main Authors: Jacquot, J., Dupuit, F., Elbtaouri, H., Hinnrasky, J., Antonicelli, F., Haye, B., Puchelle, E.
• Format: Article
• Language: English
• Subjects: Annexins; Biological and medical sciences; Calcium-Binding Proteins - biosynthesis; Calcium-Binding Proteins - isolation & purification; Cell culture; Cell physiology; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; glands; Glycoproteins - biosynthesis; Human tracheal gland; Humans; Kinetics; Lipocortin; Methionine - metabolism; Molecular and cellular biology; Molecular Weight; Mucous Membrane - metabolism; Trachea - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)81347-Q

Evidence is obtained for the presence of lipocortin-like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I. indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca 2+-dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following [ 35S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortinlike proteins with apparent M r33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by trachéal gland cells cultured on microporous membrane of a double chamber culture system.