Smooth muscle specific expression of calponin

Calponin is an actin-, calmodulin-. and tropomyosin-binding protein that has been isolated from smooth muscle tissue. Using a monoclonal antibody specific for avian calponin, we demonstrate a differentiation-linked increase in calponin expression in embryonic chick gizzard. Cultivation of gizzard sm...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1990-11, Vol.274 (1), p.159-162
Main Authors: Gimona, Mario, Herzog, Monika, Vandekerckhove, Joël, Small, John Victor
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Calcium-Binding Proteins - analysis
Calponin
Calponins
Embryo, Nonmammalian
Expression: Localization
Fluorescent Antibody Technique
Gizzard, Avian - metabolism
Immunoblotting
Microfilament Proteins
Molecular Weight
Muscle Proteins - analysis
Muscle, Smooth - metabolism
Smooth muscle
Turkeys
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Calponin is an actin-, calmodulin-. and tropomyosin-binding protein that has been isolated from smooth muscle tissue. Using a monoclonal antibody specific for avian calponin, we demonstrate a differentiation-linked increase in calponin expression in embryonic chick gizzard. Cultivation of gizzard smooth muscle cells in vitro resulted in a down-regulation of calponin expression after the first 48 h that was paralleled by a loss of synthesis of metavinculin and the high molecular weight isoform of caldesmon. In early cultures of smooth muscle cells calponin was localised in the actin-containing stress fibres but labelling was restricted to the central parts of the actin cytoskeleton. Calponin expression is suggested as a potentially useful index of smooth muscle differentiation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81353-P