Smooth muscle-like tissues engineered with bone marrow stromal cells

Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers incl...

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Bibliographic Details
Published in:Biomaterials 2004-07, Vol.25 (15), p.2979-2986
Main Authors: Cho, Seung-Woo, Kim, Il-Kwon, Lim, Sang Hyun, Kim, Dong-Ik, Kang, Sun-Woong, Kim, Soo Hyun, Kim, Young Ha, Lee, Eun Yeol, Choi, Cha Yong, Kim, Byung-Soo
Format: Article
Language:English
Subjects:
Absorbable Implants
Animals
Biodegradable polymer scaffold
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Bone marrow stromal cells
Bone Marrow Transplantation - methods
Cell Culture Techniques - methods
Cell Differentiation
Cell Division
Cells, Cultured
Dogs
Mice
Mice, Nude
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - physiology
Smooth muscle
Stromal Cells - cytology
Stromal Cells - physiology
Stromal Cells - transplantation
Tissue engineering
Tissue Engineering - methods
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Summary:Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM α-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell–scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM α-actin and SM myosin heavy chain. Masson’s trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2003.09.068