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Cytology of human ovarian surface epithelial brushings

BACKGROUND The human ovarian surface epithelium (HOSE) is the putative source of ovarian epithelial cancer, the most lethal gynecologic malignancy that affects women in the United States. The current study was designed to provide a database of normal HOSE cell features for diagnostic and research ap...

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Published in:Cancer 2004-02, Vol.102 (1), p.1-10
Main Authors: Nicosia, Santo V., Wilbanks, George D., Saunders, Beatriz, Mayer, James, Cardosi, Richard J., Kruk, Patricia A., Cheng, Jin, Bai, Wenlong, Coppola, Domenico, Fiorica, James
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cited_by cdi_FETCH-LOGICAL-c3931-398f0b53a6d8a1978e3ef6ad0b6b880d328e0932bb6fa509e2d189edfa17ac483
cites cdi_FETCH-LOGICAL-c3931-398f0b53a6d8a1978e3ef6ad0b6b880d328e0932bb6fa509e2d189edfa17ac483
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container_title Cancer
container_volume 102
creator Nicosia, Santo V.
Wilbanks, George D.
Saunders, Beatriz
Mayer, James
Cardosi, Richard J.
Kruk, Patricia A.
Cheng, Jin
Bai, Wenlong
Coppola, Domenico
Fiorica, James
description BACKGROUND The human ovarian surface epithelium (HOSE) is the putative source of ovarian epithelial cancer, the most lethal gynecologic malignancy that affects women in the United States. The current study was designed to provide a database of normal HOSE cell features for diagnostic and research applications. METHODS HOSE was harvested from 42 women undergoing laparoscopy or laparotomy for benign gynecologic disorders, infertility problems, or pregnancy. Of the 42 women, 12 were postovulatory and 20 were receiving hormonal regimens. Cells were harvested with a sterile brush inserted through a laparoscopic port or with a sterile cell scraper at laparotomy. RESULTS Two HOSE populations were identified, ranging in size from 8 to 10 μm and from 15 to 20 μm, respectively. The cells measuring 15–20 μm exhibited slight anisonucleosis, more prominent nucleoli, fine cytoplasmic metachromasia, and an overall reparative or squamoid morphology. Cells were single or arranged in small clusters, sheets, or papillae. They coexpressed cytokeratin and vimentin but did not overexpress p53. Cellularity and proliferation (up to 3.2% ± 0.8) were higher and papillae more frequent in postovulatory and cyst‐bearing ovaries, including polycystic ovaries, suggesting underlying ovarian or hormonal influences. Representative HOSE brushings yielded a mean of 23,133 cells per patient (range, 4250–64,500 cells), equivalent to an estimated 0.58, 0.46, and 0.14 μg of nuclear protein, cell RNA, and nuclear DNA, respectively. Within 7–10 days of explantation, HOSE cells formed confluent monolayers with immunohistochemical and ultrastructural epithelial features. CONCLUSIONS The current study defined baseline features of HOSE cells important to pathologists and clinicians evaluating women at risk for ovarian epithelial cancer and to researchers investigating the pathobiology of this aggressive gynecologic malignancy. Cancer (Cancer Cytopathol) 2004;102:1–10. © 2003 American Cancer Society. The current study defined baseline features of human ovarian surface epithelial (HOSE) cells. The authors demonstrated that HOSE cells are important to pathologists and clinicians evaluating women at risk for ovarian epithelial cancer and to researchers investigating the pathobiology of this aggressive gynecologic malignancy.
doi_str_mv 10.1002/cncr.20001
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The current study was designed to provide a database of normal HOSE cell features for diagnostic and research applications. METHODS HOSE was harvested from 42 women undergoing laparoscopy or laparotomy for benign gynecologic disorders, infertility problems, or pregnancy. Of the 42 women, 12 were postovulatory and 20 were receiving hormonal regimens. Cells were harvested with a sterile brush inserted through a laparoscopic port or with a sterile cell scraper at laparotomy. RESULTS Two HOSE populations were identified, ranging in size from 8 to 10 μm and from 15 to 20 μm, respectively. The cells measuring 15–20 μm exhibited slight anisonucleosis, more prominent nucleoli, fine cytoplasmic metachromasia, and an overall reparative or squamoid morphology. Cells were single or arranged in small clusters, sheets, or papillae. They coexpressed cytokeratin and vimentin but did not overexpress p53. Cellularity and proliferation (up to 3.2% ± 0.8) were higher and papillae more frequent in postovulatory and cyst‐bearing ovaries, including polycystic ovaries, suggesting underlying ovarian or hormonal influences. Representative HOSE brushings yielded a mean of 23,133 cells per patient (range, 4250–64,500 cells), equivalent to an estimated 0.58, 0.46, and 0.14 μg of nuclear protein, cell RNA, and nuclear DNA, respectively. Within 7–10 days of explantation, HOSE cells formed confluent monolayers with immunohistochemical and ultrastructural epithelial features. CONCLUSIONS The current study defined baseline features of HOSE cells important to pathologists and clinicians evaluating women at risk for ovarian epithelial cancer and to researchers investigating the pathobiology of this aggressive gynecologic malignancy. Cancer (Cancer Cytopathol) 2004;102:1–10. © 2003 American Cancer Society. The current study defined baseline features of human ovarian surface epithelial (HOSE) cells. 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The current study was designed to provide a database of normal HOSE cell features for diagnostic and research applications. METHODS HOSE was harvested from 42 women undergoing laparoscopy or laparotomy for benign gynecologic disorders, infertility problems, or pregnancy. Of the 42 women, 12 were postovulatory and 20 were receiving hormonal regimens. Cells were harvested with a sterile brush inserted through a laparoscopic port or with a sterile cell scraper at laparotomy. RESULTS Two HOSE populations were identified, ranging in size from 8 to 10 μm and from 15 to 20 μm, respectively. The cells measuring 15–20 μm exhibited slight anisonucleosis, more prominent nucleoli, fine cytoplasmic metachromasia, and an overall reparative or squamoid morphology. Cells were single or arranged in small clusters, sheets, or papillae. They coexpressed cytokeratin and vimentin but did not overexpress p53. Cellularity and proliferation (up to 3.2% ± 0.8) were higher and papillae more frequent in postovulatory and cyst‐bearing ovaries, including polycystic ovaries, suggesting underlying ovarian or hormonal influences. Representative HOSE brushings yielded a mean of 23,133 cells per patient (range, 4250–64,500 cells), equivalent to an estimated 0.58, 0.46, and 0.14 μg of nuclear protein, cell RNA, and nuclear DNA, respectively. Within 7–10 days of explantation, HOSE cells formed confluent monolayers with immunohistochemical and ultrastructural epithelial features. CONCLUSIONS The current study defined baseline features of HOSE cells important to pathologists and clinicians evaluating women at risk for ovarian epithelial cancer and to researchers investigating the pathobiology of this aggressive gynecologic malignancy. Cancer (Cancer Cytopathol) 2004;102:1–10. © 2003 American Cancer Society. The current study defined baseline features of human ovarian surface epithelial (HOSE) cells. 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The current study was designed to provide a database of normal HOSE cell features for diagnostic and research applications. METHODS HOSE was harvested from 42 women undergoing laparoscopy or laparotomy for benign gynecologic disorders, infertility problems, or pregnancy. Of the 42 women, 12 were postovulatory and 20 were receiving hormonal regimens. Cells were harvested with a sterile brush inserted through a laparoscopic port or with a sterile cell scraper at laparotomy. RESULTS Two HOSE populations were identified, ranging in size from 8 to 10 μm and from 15 to 20 μm, respectively. The cells measuring 15–20 μm exhibited slight anisonucleosis, more prominent nucleoli, fine cytoplasmic metachromasia, and an overall reparative or squamoid morphology. Cells were single or arranged in small clusters, sheets, or papillae. They coexpressed cytokeratin and vimentin but did not overexpress p53. Cellularity and proliferation (up to 3.2% ± 0.8) were higher and papillae more frequent in postovulatory and cyst‐bearing ovaries, including polycystic ovaries, suggesting underlying ovarian or hormonal influences. Representative HOSE brushings yielded a mean of 23,133 cells per patient (range, 4250–64,500 cells), equivalent to an estimated 0.58, 0.46, and 0.14 μg of nuclear protein, cell RNA, and nuclear DNA, respectively. Within 7–10 days of explantation, HOSE cells formed confluent monolayers with immunohistochemical and ultrastructural epithelial features. CONCLUSIONS The current study defined baseline features of HOSE cells important to pathologists and clinicians evaluating women at risk for ovarian epithelial cancer and to researchers investigating the pathobiology of this aggressive gynecologic malignancy. Cancer (Cancer Cytopathol) 2004;102:1–10. © 2003 American Cancer Society. The current study defined baseline features of human ovarian surface epithelial (HOSE) cells. 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source Wiley; EZB Electronic Journals Library
subjects Adult
Aged
Biological and medical sciences
Biopsy, Needle
cell yield
Cohort Studies
Cytodiagnosis
cytology
Epithelial Cells - pathology
Female
human ovarian surface epithelium
Humans
Immunohistochemistry
Laparoscopy
Laparotomy
Medical sciences
Middle Aged
Neoplasms, Glandular and Epithelial - pathology
Neoplasms, Glandular and Epithelial - surgery
nucleic acid/protein content
ovarian brushings
Ovarian Diseases - pathology
Ovarian Diseases - surgery
Ovarian Neoplasms - pathology
Ovarian Neoplasms - surgery
Sensitivity and Specificity
Tumor Cells, Cultured
Tumors
title Cytology of human ovarian surface epithelial brushings
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