Controlled secretion of β-endorphin from human embryonic kidney cells carrying a Tet-on-β-endorphin fusion gene

To create a cell line with controlled and specific secretion of β-endorphin, a new fusion gene was constructed by joining human β-endorphin coding sequence to part of NL1 gene. HEK293 cells carrying Tet-on system transfected with this fusion gene secreted β-endorphin in a dose-dependent manner upon...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 2004-02, Vol.121 (1), p.151-155
Main Authors: Saitoh, Youichi, Eguchi, Yutaka, Nakahira, Rie, Yasuda, Keitaro, Moriuchi, Shu-ichi, Yoshimine, Toshiki, Boileau, Guy
Format: Article
Language:English
Subjects:
beta-Endorphin - secretion
Biological and medical sciences
Cell Line
Dose-Response Relationship, Drug
Doxycycline - pharmacology
Embryo, Mammalian - cytology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Genetic Engineering
Humans
Kidney - cytology
Pain
Pro-Opiomelanocortin - genetics
Promoter Regions, Genetic
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - physiology
Tet-on system
Tetracycline
Tetracycline - pharmacology
Transcriptional Activation - drug effects
Transfection
Vertebrates: nervous system and sense organs
β-Endorphin
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Summary:To create a cell line with controlled and specific secretion of β-endorphin, a new fusion gene was constructed by joining human β-endorphin coding sequence to part of NL1 gene. HEK293 cells carrying Tet-on system transfected with this fusion gene secreted β-endorphin in a dose-dependent manner upon doxycycline administration. These findings suggest that this system can direct the controlled secretion of any peptide hormones such as β-endorphin.
ISSN:0169-328X
1872-6941
DOI:10.1016/j.molbrainres.2003.11.010