RECOMBINANT SENDAI VIRUS FOR EFFICIENT GENE TRANSFER TO HUMAN AIRWAY EPITHELIUM

Recombinant Sendai virus (rSeV) infects respiratory epithelial cells in animal models and cultures of undifferentiated human nasal cells. It was the aim of this study to investigate the capability of rSeV to express a transgene in human airway epithelium. Differentiated human airway epithelial cells...

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Bibliographic Details
Published in:Experimental lung research 2004, Vol.30 (2), p.83-96
Main Authors: Pinkenburg, Olaf, Vogelmeier, Claus, Bossow, Sascha, Neubert, Wolfgang J., Lutz, Raimund B., Ungerechts, Guy, Lauer, Ulrich M., Bitzer, Michael, Bals, Robert
Format: Article
Language:English
Subjects:
airway epithelium
Biological and medical sciences
Bronchi - metabolism
Culture Techniques
cystic fibrosis
Epithelium - metabolism
Gene Expression
gene therapy
Gene Transfer Techniques
Genetic Vectors
Green Fluorescent Proteins
Humans
Inflammation - etiology
Luminescent Proteins - genetics
Medical sciences
Pneumology
Receptors, Cell Surface - metabolism
Recombinant Proteins - genetics
Recombination, Genetic
Sendai virus
Sendai virus - genetics
Sendai virus - pathogenicity
Sendai virus - physiology
Virus Replication
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Summary:Recombinant Sendai virus (rSeV) infects respiratory epithelial cells in animal models and cultures of undifferentiated human nasal cells. It was the aim of this study to investigate the capability of rSeV to express a transgene in human airway epithelium. Differentiated human airway epithelial cells were generated using air-liquid interface culture techniques. Application of rSeV coding for green fluorescence protein (GFP) onto the apical surface (using a multiplicity of infection of 3) resulted in expression of the transgene in more than 90% of the cells followed by decreasing numbers of positive cells during the observation time of 3 weeks. The infection of human respiratory epithelial cells is mediated by sialic acid residues at the apical surface. Despite the secretion of interleukin (IL)-8 and the replication of rSeV in the epithelial cells, the authors could not detect any cytopathic effect after the infection. In conclusion, rSeV infects differentiated human airway epithelial cells with high efficiency. Transgene expression is transient and accompanied by the secretion of an inflammatory cytokine.
ISSN:0190-2148
1521-0499
DOI:10.1080/01902140490266501