Genetic diversity and insecticide resistance of Myzus persicae (Hemiptera: Aphididae) populations from tobacco in Chile: evidence for the existence of a single predominant clone

The tobacco-feeding race of Myzus persicae (Sulzer), formerly known as M. nicotianae Blackman, was introduced into Chile during the last decade. In order to evaluate the genetic diversity and insecticide resistance status of Chilean tobacco aphid populations, a field survey was conducted in 35 tobac...

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Bibliographic Details
Published in:Bulletin of entomological research 2004-02, Vol.94 (1), p.11-18
Main Authors: Fuentes-Contreras, E., Figueroa, C.C., Reyes, M., Briones, L.M., Niemeyer, H.M.
Format: Article
Language:English
Subjects:
Animals
Aphididae
Aphidoidea
Aphids - drug effects
Aphids - genetics
Biological and medical sciences
Chemical control
Chile
clones
Cloning
Control
enzyme activity
esterases
Female
Fundamental and applied biological sciences. Psychology
Genetic diversity
Genetic Variation
Genotype
insect morphology
insecticide resistance
Insecticide Resistance - genetics
Insecticides
Insects
kdr mutation
Male
Microsatellite Repeats
morphometry
Multivariate analysis
Mutation
Myzus persicae
Nicotiana
Nicotiana - parasitology
Nicotiana tabacum
Organophosphates
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Pyrethroids
sodium channels
Tobacco
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Summary:The tobacco-feeding race of Myzus persicae (Sulzer), formerly known as M. nicotianae Blackman, was introduced into Chile during the last decade. In order to evaluate the genetic diversity and insecticide resistance status of Chilean tobacco aphid populations, a field survey was conducted in 35 tobacco fields covering a 300 km latitudinal survey. The populations sampled were characterized using microsatellite markers and morphometric multivariate analysis. Insecticide resistance levels were assessed through a microplate esterase assay and the mutation status of the kdr gene. All samples collected corresponded to the same anholocyclic aphid genotype, and showed morphological variation within the range expected for the tobacco-feeding race of M. persicae. Esterase activity showed the level and variability expected for an R1 clone lacking mutations in the sodium channels (susceptible kdr), thus corresponding to a type slightly resistant to organophosphate and carbamate, and susceptible to pyrethroid insecticides.
ISSN:0007-4853
1475-2670
DOI:10.1079/BER2003275