Tsga10 Encodes a 65-Kilodalton Protein That Is Processed to the 27-Kilodalton Fibrous Sheath Protein

• Published in: Biology of reproduction 2004-03, Vol.70 (3), p.608-615
• Main Authors: MODARRESSI, Mohammad H, BEHNAM, Babak, MIN CHENG, TAYLOR, Kay E, WOLFE, Jonathan, VAN DER HOORN, Frans A
• Format: Article
• Language: English
• Subjects: 5' Flanking Region - genetics; Animals; Base Sequence; Biological and medical sciences; Exons; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Male; Mammalian male genital system; Mice; Molecular Sequence Data; Morphology. Physiology; Myosins - chemistry; Myosins - genetics; NIH 3T3 Cells; Protein Biosynthesis; Protein Structure, Tertiary; Proteins - chemistry; Proteins - genetics; RNA, Messenger - analysis; Seminal Plasma Proteins - chemistry; Seminal Plasma Proteins - genetics; Sperm Tail; Spermatids - physiology; Spermatogenesis - physiology; Testicular Neoplasms - physiopathology; Testis - physiology; Vertebrates: reproduction
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod.103.021170

We had previously reported the isolation of the testis-specific human gene Tsga 10, which is not expressed in testes from two infertile patients. To study its role and function, we cloned the mouse homologue Mtsga 10. Mtsga 10 localizes to mouse chromosome 1, band B. This region is syntenic with human chromosome 2q11.2, where Tsga 10 is located. We demonstrate that Mtsga10 mRNA is expressed in testis, but not in other adult tissues, and in several human fetal tissues and primary tumors. We uncovered that different species use different first exons and, consequently, different promoters. Using several antibodies, we discovered that, in mouse testis, Mtsga 10 encodes a 65-kDa spermatid protein that appears to be processed to a 27-kDa protein of the fibrous sheath, a major sperm tail structure, in mature spermatozoa. Mtsga10 protein contains a putative myosin/Ezrin/radixin/moesin (ERM) domain. Transfection of fibroblasts with GFP-Mtsga10 fusion protein results in formation of short, thick filaments and deletion of the myosin/ERM domain abolished filament formation. Our results suggest the possibility that Tsga10 plays a role in the sperm tail fibrous sheath.