Evidence that the anti-obesity effect of conjugated linoleic acid is independent of effects on stearoyl-CoA desaturase1 expression and enzyme activity

The trans-10, cis-12 isomer of conjugated linoleic acid (CLA) reduces body fat gain in animals and inhibits stearoyl-CoA desaturase (SCD) activity in 3T3-L1 adipocytes. To test whether CLA’s body fat reduction is mediated by SCD1, wild-type and SCD1-null mice were fed diet supplemented with 0.2% tra...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-03, Vol.315 (3), p.532-537
Main Authors: Kang, Kihwa, Miyazaki, Makoto, Ntambi, James M, Pariza, Michael W
Format: Article
Language:English
Subjects:
3-Hydroxybutyric Acid - blood
Adipose tissue
Adipose Tissue - chemistry
Adipose Tissue - metabolism
Animal Feed
Animals
Anti-Obesity Agents - chemistry
Anti-Obesity Agents - pharmacology
Blood Glucose - analysis
Body Weight - drug effects
Cholesterol - blood
Conjugated linoleic acid
Fats - analysis
Fatty Acids - analysis
Fatty Acids - chemistry
Gene Expression
Linoleic Acid - chemistry
Linoleic Acid - pharmacology
Liver - chemistry
Male
Mice
Mice, Knockout
Muscles - chemistry
RNA, Messenger - biosynthesis
Stearoyl-CoA desaturase
Stearoyl-CoA Desaturase - deficiency
Stearoyl-CoA Desaturase - genetics
Stearoyl-CoA Desaturase - metabolism
Trans-10, cis-12 CLA
Triglycerides - blood
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Summary:The trans-10, cis-12 isomer of conjugated linoleic acid (CLA) reduces body fat gain in animals and inhibits stearoyl-CoA desaturase (SCD) activity in 3T3-L1 adipocytes. To test whether CLA’s body fat reduction is mediated by SCD1, wild-type and SCD1-null mice were fed diet supplemented with 0.2% trans-10, cis-12 (t10c12) CLA for 4 weeks. The t10c12 CLA-supplemented diet significantly reduced body fat mass in both wild type and SCD1-null mice. Similarly, t10c12 CLA diet decreased blood triglyceride and free fatty acid levels regardless of SCD1 genotypes. Mice fed t10c12 CLA exhibited increased mRNA expression of fatty acid synthase and uncoupling protein 2 in both genotypes. Taken together, the effects of t10c12 CLA on reduction of body fat gain, blood parameters, and mRNA expression in both SCD1-null mice and wild-type mice were similar, indicating that the anti-obesity effect of t10c12 CLA may be independent of the effects of this CLA isomer on SCD1 gene expression and enzyme activity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.01.087