Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes

Recently there has been interest in developing cell and gene therapies with adult stem cells from human bone marrow referred to as mesenchymal stem cells or marrow stromal cells (hMSCs). We incubated early-passage hMSCs in serum-free medium without cytokines or other supplements for 2 to 4 weeks. Su...

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Bibliographic Details
Published in:Blood 2004-03, Vol.103 (5), p.1647-1652
Main Authors: Pochampally, Radhika R., Smith, Jason R., Ylostalo, Joni, Prockop, Darwin J.
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Applied cell therapy and gene therapy
Biological and medical sciences
Blotting, Western
Bone Marrow Cells - cytology
Cell Culture Techniques - methods
Cell Differentiation
Cell Division
Culture Media, Serum-Free - pharmacology
DNA-Binding Proteins - biosynthesis
Down-Regulation
Humans
Medical sciences
Microscopy, Phase-Contrast
Octamer Transcription Factor-3
Oligonucleotide Array Sequence Analysis
Other treatments
Reverse Transcriptase Polymerase Chain Reaction
RNA - metabolism
Stem Cells - cytology
Stromal Cells - cytology
Telomere - ultrastructure
Time Factors
Transcription Factors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Treatment. General aspects
Tumors
Up-Regulation
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Summary:Recently there has been interest in developing cell and gene therapies with adult stem cells from human bone marrow referred to as mesenchymal stem cells or marrow stromal cells (hMSCs). We incubated early-passage hMSCs in serum-free medium without cytokines or other supplements for 2 to 4 weeks. Surprisingly, a subpopulation of the cells survived serum deprivation and then began to proliferate in serum-containing medium. The cells selected by serum deprivation had longer telomeres than control cells. Also, the patterns of gene expression revealed by reverse transcriptase–polymerase chain reaction (RT-PCR) assays and microarray data indicated that the cells selected by serum deprivation were a subpopulation of very early progenitor cells with enhanced expression of octomer-binding transcription factor 4 (OCT-4) and several other genes characteristically expressed in embryonic cells.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2003-06-1967