Coils and tubes releasing heparin. Studies on a new vascular graft prototype

• Published in: Biomaterials 2004-07, Vol.25 (16), p.3125-3133
• Main Authors: Aldenhoff, Yvette B.J., Knetsch, Menno L.W., Hanssen, Johannes H.L., Lindhout, Theo, Wielders, Simone J.H., Koole, Leo H.
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Animals; Blood compatibility; Blood Vessel Prosthesis; Catheterization - instrumentation; Catheterization - methods; Cell Adhesion - drug effects; Cell Division - drug effects; Cell Line; Coated Materials, Biocompatible - administration & dosage; Coated Materials, Biocompatible - chemistry; Drug Delivery Systems - instrumentation; Drug Delivery Systems - methods; Endothelial cells; Endothelial Cells - cytology; Endothelial Cells - drug effects; Endothelial Cells - physiology; Equipment Failure Analysis; Heparin; Heparin - administration & dosage; Heparin - chemistry; Humans; Materials Testing; Mice; Pilot Projects; Prosthesis Design; Thrombogenicity; Thrombosis - prevention & control; Transplants; Vascular grafts
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2003.10.012

Coiled metallic guidewires find widespread use, for instance in interventional cardiology. It is known that release of heparin from the surface of guidewires is advantageous to prevent formation of thrombotic emboli. New coiled tubular structures, having larger inner and outer diameter as compared to guidewires, are presented. In theory these tubes can be used as interposition vascular grafts. Ten coiled tubes with an internal diameter of 690 μm were made. Five different adherent polymeric coatings with increasing hydrophilicity were used. Five tubes contained heparin in the coating and the other five were unheparinised controls. The five tubes containing heparin were studied with respect to heparin release in vitro (amount released, kinetics), and immobilised heparin that is exposed at the surface. All tubes were studied with a direct cell contact assay using 3T3 mouse fibroblast cells, a dynamic thrombin generation test, and endothelial cell growth onto the coils. It was found that the heparinised tubes lead to very little thrombin formation. It is argued that this is due to heparin that is immobilised and exposed at the inner surface of such tubes. Furthermore the coils showed to be cytocompatible and endothelial cells adhere and proliferate well onto the coils. This concept is believed to hold promise for further development of small vascular grafts.