Electrophoretic separation by an improved method of fast myosin HCIIb-,HCIId-, and HCIIa-based isomyosins with specific alkali light chain combinations

An improved method of electrophoresis under nondenaturing conditions separated three electrophoretically distinct isomyosin triplets when applied to rat fast-twitch muscles displaying a predominance of one of the fast myosin heavy chain HCIIb, HCIId or HCIIa. The three isomyosin triplets, named FM1b...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1990-11, Vol.275 (1), p.165-167
Main Authors: Termin, Angelika, Pette, Dirk
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Contractile proteins
Electrophoresis - methods
Fundamental and applied biological sciences. Psychology
Holoproteins
Isoenzymes - analysis
Isomyosin electrophoresis
Male
Muscles - enzymology
myosin
Myosin alkali light chain
Myosin heavy chain
Myosin heavy chain-based isomyosin
Myosins - analysis
Proteins
Rats
Rats, Inbred Strains
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An improved method of electrophoresis under nondenaturing conditions separated three electrophoretically distinct isomyosin triplets when applied to rat fast-twitch muscles displaying a predominance of one of the fast myosin heavy chain HCIIb, HCIId or HCIIa. The three isomyosin triplets, named FM1b-FM3b, FM1d-FM3d, FM1a-FM3a, correspond to the three possible alkali light chain (I.C.) combinations (L.C.??? homodimer, LC??? LC3f heterodimer, and LC3f homodimer) with each fast HC isoform. Different proportions of these various isomyosins suggested specific affinities of light chains LC??? and LC3f for the fast heavy chain isoforms.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81463-X