HPLC-PAD-APCI/MS assay of phenylpropanoids in cereals

A new, rapid HPLC-PAD-APCI/MS assay has been developed in order to measure accurately the amount of p-coumaric, E- and Z-ferulic acid and the dehydrodimers of ferulic acid in cereal grain. In the positive ionisation mode, MS patterns gave additional information for the identification of the dimers....

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Bibliographic Details
Published in:Phytochemical analysis 2004, Vol.15 (1), p.9-15
Main Authors: Bily, A.C, Burt, A.J, Ramputh, A.I, Livesey, J, Regnault-Roger, C, Philogene, B.R, Arnason, J.T
Format: Article
Language:English
Subjects:
cell wall
cell walls
cereals
chemical analysis
Chromatography, High Pressure Liquid - methods
corn
coumaric acids
Coumaric Acids - analysis
diferulates
Edible Grain - chemistry
Gas Chromatography-Mass Spectrometry - methods
grain crops
high performance liquid chromatography
HPLC-PAD-APCI/MS
hydroxycinnamates
mass spectrometry
Oryza - chemistry
pericarp
phenylpropanoids
phytochemicals
Propionates
Sorghum - chemistry
Triticum - chemistry
Zea mays
Zea mays - chemistry
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Summary:A new, rapid HPLC-PAD-APCI/MS assay has been developed in order to measure accurately the amount of p-coumaric, E- and Z-ferulic acid and the dehydrodimers of ferulic acid in cereal grain. In the positive ionisation mode, MS patterns gave additional information for the identification of the dimers. The time required and the quantities of solvents employed in the developed analytical method are much lower than those involved in previously available assays of these compounds, thus making the method suitable for the screening of cereal genotypes. Application of the method to accessions of maize, wheat and sorghum showed that E-ferulic was the most abundant phenylpropanoid, whilst the major dimer was 8-O-4' dehydrodimer of ferulic acid followed by the 5-5' and then the 8-5' forms. Maize grains, especially of the Mexican landraces, contained the highest levels of these dimers.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.735