Identification of Polypeptide Precursors to HSV-1 Glycoproteins by Cell-free Translation

Department of Biochemistry University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K. Using antisera of limited specificity we have detected among the in vitro translation products of HSV-1 RNA, polypeptides antigenically related to infected cell glycoproteins. The results obtained suggest...

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Bibliographic Details
Published in:Journal of general virology 1982-01, Vol.58 (1), p.217-222
Main Authors: Inglis, Moira M, Newton, Alison A
Format: Article
Language:English
Subjects:
Cell-Free System
Electrophoresis, Polyacrylamide Gel
Glycoproteins - biosynthesis
herpes simplex virus 1
Molecular Weight
Protein Biosynthesis
Protein Precursors - isolation & purification
Simplexvirus - analysis
Viral Proteins - biosynthesis
Viral Proteins - isolation & purification
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Summary:Department of Biochemistry University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K. Using antisera of limited specificity we have detected among the in vitro translation products of HSV-1 RNA, polypeptides antigenically related to infected cell glycoproteins. The results obtained suggest that non-glycosylated polypeptide precursors of mol. wt. 85000 and 52000 correspond to infected cell glycoproteins of mol. wt. 120000 to 126000 and 56000 to 68000 respectively. Keywords: HSV-1, glycoproteins, non-glycosylated precursors, cell-free translation Present address: Division of Virology, Department of Pathology, University of Cambridge, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, U.K. Received 11 June 1981; accepted 11 August 1981.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-58-1-217