In vitro Estrogenicity of Resin Composites

Previously, we have reported that sealants incorporating bisphenol A dimethacrylate showed estrogenicity by a reporter gene assay. This study tested the hypothesis that commercial composites, which contain various monomers and additives, exhibit estrogenic activity in vitro. The estrogenic activitie...

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Bibliographic Details
Published in:Journal of dental research 2004-03, Vol.83 (3), p.222-226
Main Authors: Wada, H., Tarumi, H., Imazato, S., Narimatsu, M., Ebisu, S.
Format: Article
Language:English
Subjects:
Acetophenones - analysis
Acetophenones - pharmacology
Analysis of Variance
Benzophenones - analysis
Benzophenones - pharmacology
Butylated Hydroxytoluene - analysis
Butylated Hydroxytoluene - pharmacology
Cell Line
Chromatography, High Pressure Liquid
Composite Resins - analysis
Composite Resins - pharmacology
Dentistry
Estrogens - agonists
Genes, Reporter - genetics
Humans
Plasmids
Receptors, Estrogen - agonists
Transfection
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Summary:Previously, we have reported that sealants incorporating bisphenol A dimethacrylate showed estrogenicity by a reporter gene assay. This study tested the hypothesis that commercial composites, which contain various monomers and additives, exhibit estrogenic activity in vitro. The estrogenic activities of eluates obtained from 24 composites and 18 chemicals identified from the composites tested were examined with the use of the reporter gene assay. Among the 24 composites, 6 products were estrogenic, and among the 18 constituents, 1 photostabilizer, 2-hydroxy-4-methoxy-benzophenone (HMBP), 1 photoinitiator, 2,2-dimethoxy-2-phenyl-acetophenone (DMPA), and 1 inhibitor, 2,6-di-tert-butyl-p-cresol (BHT) had significant estrogenic activity. The concentration of HMBP in 4 estrogenic eluates was greater than the minimum concentration required for estrogenicity, and DMPA was found at a higher level than the minimum estrogenic concentration in the remaining 2 estrogenic specimens. These results suggest that the observed estrogenic activity of 6 composites is associated with the elution of either HMBP or DMPA.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910408300307