Triacylglycerol lipase from rape (Brassica napus L.) suitable for biotechnological purposes

Triacylglycerol lipase (EC 3.1.1.3) from rape (Brassica napus L. cv Ceres) is quite easily prepared from the 100,000 x g supernatant of cotyledon homogenates. The lipase is present in a high-molecular-mass fraction (greater than 1.5 x 10(6) dalton by gel filtration), but it can be rapidly extracted...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1990-10, Vol.26 (1), p.1-10
Main Authors: Hills, M.J. (H.P. Kaufmann-Institut, Munster, Federal Republic of Germany), Mukherjee, K.D
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
activity
applications
biotechnology
Brassica - enzymology
BRASSICA NAPUS
characterization
Chemical Precipitation
COTILEDONES
COTYLEDON
COTYLEDONS
enzymatic synthesis
Enzymes, Immobilized
ENZYMIC ACTIVITY
ESTERIFICACION
ESTERIFICATION
IMMOBILISATION
IMMOBILIZATION
immobilized enzymes
INMOVILIZACION
ISOLATION
Lipase - isolation & purification
Lipase - metabolism
Molecular Weight
Oleic Acid
Oleic Acids - metabolism
purification
Substrate Specificity
TRIACILGLICEROL LIPASA
TRIACYLGLYCEROL LIPASE
Triglycerides - metabolism
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Description
Summary:Triacylglycerol lipase (EC 3.1.1.3) from rape (Brassica napus L. cv Ceres) is quite easily prepared from the 100,000 x g supernatant of cotyledon homogenates. The lipase is present in a high-molecular-mass fraction (greater than 1.5 x 10(6) dalton by gel filtration), but it can be rapidly extracted from the 100,000 x g supernatant by precipitation with polyethyleneglycol 8000 (4%, w/v) and MgCl2 (40 mM) giving about a 10-fold purification. After delipidation, the lipase has an Mr of about 300,000. It hydrolyzes triacylglycerols to fatty acids and glycerol, although the fatty acids from the sn-1 or -3 positions are hydrolyzed first to yield 1,2(2,3)-diacylglycerols. Lipase immobilized onto Celite by precipitation with acetone at -20 degrees C catalyzes the esterification of oleic acid with butanol dissolved in hexane.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02798388