N-Laurylbiotinamide as Affinity Surfactant

N‐laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N‐laurylamine via the biotin ester of N‐hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isoo...

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Bibliographic Details
Published in:Biotechnology progress 1990-07, Vol.6 (4), p.307-309
Main Authors: Coughlin, Robert W., Baclaski, Jeffrey B.
Format: Article
Language:English
Subjects:
Avidin - chemistry
Avidin - isolation & purification
Biotechnology
Biotin - analogs & derivatives
Biotin - chemical synthesis
Biotin - chemistry
Chemical Phenomena
Chemistry, Physical
Dioctyl Sulfosuccinic Acid
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Micelles
Octanes
Solutions
Surface-Active Agents
Water
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Summary:N‐laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N‐laurylamine via the biotin ester of N‐hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse‐micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n‐octyl β‐D‐glucopyranoside.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp00004a012