Cell culture amplification of a defective Marek's disease virus

A highly amplified 4-kb EcoRI fragment was present in DNA isolated from high cell culture passaged stocks (greater than 93 passages) of 281MI/1, a serotype 2 Marek's disease virus (MDV). The isolated 4-kb fragment is amplified in the presence of MDV, replicating as a high molecular weight, head...

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Bibliographic Details
Published in:Virus genes 1990-09, Vol.4 (3), p.225-237
Main Authors: Carter, J.K. (Regional Poultry Research Lab. USDA-ARS, East Lansing, MI (USA)), Silva, R.F
Format: Article
Language:English
Subjects:
ADN
AVES DE CORRAL
CELL CULTURE
CHICKENS
CLONACION
CLONAGE
CLONING
Cloning, Molecular
CULTIVO DE CELULAS
CULTURE DE CELLULE
DEFAUT
DEFECTOS
DEFECTS
DNA
DNA - analysis
DNA Probes
Gene Amplification
Genes, Viral
Herpesvirus 2, Gallid - genetics
HERPETOVIRIDAE
POLLO
Polymorphism, Restriction Fragment Length
POULET
POULTRY
Restriction Mapping
SEROTIPOS
SEROTYPE
SEROTYPES
Transfection
VECTEUR DE MALADIE
VECTORES
VECTORS
VOLAILLE
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Description
Summary:A highly amplified 4-kb EcoRI fragment was present in DNA isolated from high cell culture passaged stocks (greater than 93 passages) of 281MI/1, a serotype 2 Marek's disease virus (MDV). The isolated 4-kb fragment is amplified in the presence of MDV, replicating as a high molecular weight, head-to-tail concatemer. When the 4-kb fragment was cloned into pUC18 and cotransfected with MDV DNA into chicken embryo fibroblast cells, the plasmid clone also replicated as a high molecular weight concatemer.
ISSN:0920-8569
1572-994X
DOI:10.1007/BF00265632