Genetic Variation of Japanese Encephalitis Virus in Nature

Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, P.O. Box 3333, New Haven, Connecticut 06510, U.S.A. Forty-six strains of Japanese encephalitis (JE) virus from a variety of geographic areas in Asia were examined by primer-extension seque...

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Bibliographic Details
Published in:Journal of general virology 1990-12, Vol.71 (12), p.2915-2922
Main Authors: Chen, Woan-Ru, Tesh, Robert B, Rico-Hesse, Rebeca
Format: Article
Language:English
Subjects:
Animals
Asia
Base Sequence
Biological and medical sciences
Encephalitis Virus, Japanese - genetics
Encephalitis Virus, Japanese - isolation & purification
Epidemiology
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic Variation
Geography
Humans
Microbiology
Molecular Sequence Data
Phylogeny
RNA, Viral - genetics
RNA, Viral - isolation & purification
Virology
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Summary:Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, P.O. Box 3333, New Haven, Connecticut 06510, U.S.A. Forty-six strains of Japanese encephalitis (JE) virus from a variety of geographic areas in Asia were examined by primer-extension sequencing of the RNA template. A 240 nucleotide sequence from the pre-M gene region was selected for study because it provided sufficient information for determining genetic relationships among the virus isolates. Using 12% divergence as a cutoff point for virus relationships, the 46 isolates fell into three distinct genotypic groups. One genotypic group consisted of JE virus isolates from northern Thailand and Cambodia. A second group was composed of isolates from southern Thailand, Malaysia, Sarawak and Indonesia. The remainder of the isolates, from Japan, China, Taiwan, the Philippines, Sri Lanka, India and Nepal, made up a third group. The implications of these findings in relation to the epidemiology of JE are discussed. Results of this study demonstrate that the comparison of short nucleotide sequences can provide insight into JE virus evolution, transmission and, possibly, pathogenesis. Received 15 May 1990; accepted 23 August 1990.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-71-12-2915