Presence of adrenal medullary chromaffin cells in primary adrenal dispersions and their persistence in long-term culture

Cell suspensions prepared by enzymatic dispersion of whole rat adrenal glands for the purpose of studying adrenocortical cells were found to contain chromaffin cells even though they are commonly thought to not survive in such preparations. These cells fluoresced when treated with methods specific f...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 1982-01, Vol.227 (1), p.225-229
Main Authors: Moore, N A, Boler, R K, Bazer, G T
Format: Article
Language:English
Subjects:
Adrenal Cortex - cytology
Adrenal Medulla - analysis
Adrenal Medulla - cytology
Animals
Catecholamines - analysis
Cell Separation
Cell Survival
Cells, Cultured - cytology
Female
Male
Rats
Rats, Inbred Strains
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell suspensions prepared by enzymatic dispersion of whole rat adrenal glands for the purpose of studying adrenocortical cells were found to contain chromaffin cells even though they are commonly thought to not survive in such preparations. These cells fluoresced when treated with methods specific for catecholamines. The fluorescent cells persisted in the cultures of "cortical" cells, took on the morphology of neurons in the cultures, maintained their specific catecholamine fluorescence in long-term cultures, and ultrastructurally were identical to chromaffin cells.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00206344