Genetic basis of the neonatal antibody repertoire: germline V-gene expression and limited N-region diversity

We report here on the molecular characterization of heavy and light chain V-reglons of antibodies isolated from the highly connected idiotypic network of the newborn BALB/c mouse. Nucleotide sequence analysis of eight hybridomas confirmed their germline origin. Furthermore, In contrast to most hybri...

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Bibliographic Details
Published in:International immunology 1990, Vol.2 (7), p.639-643
Main Authors: Carisson, Leif, Holmberg, Dan
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antibody Diversity - genetics
B-Lymphocytes - immunology
Base Sequence
DNA - genetics
Genes, Immunoglobulin
germline antibodies
Hybridomas - immunology
idiotypic connectivity
Mice
Molecular Sequence Data
N-region diversity
neonatal antibodies
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Summary:We report here on the molecular characterization of heavy and light chain V-reglons of antibodies isolated from the highly connected idiotypic network of the newborn BALB/c mouse. Nucleotide sequence analysis of eight hybridomas confirmed their germline origin. Furthermore, In contrast to most hybridomas and myelomas derived from adult mice, the majority of these clones were found to lack N-region sequences. These data show that somatic processes amplifying junctlonal diversity are relatively inactive early in ontogeny, and that germline gene expression alone ensures Idiotypic complementarities in the developing Immune system.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/2.7.639