Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation

An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) we...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1990, Vol.34(8), pp.703-708
Main Authors: Hamashima, Hajime, Nakano, Toshikazu, Tamura, Shinobu, Arai, Taketoshi
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biotechnology
DNA
DNA, Bacterial - genetics
DNA, Recombinant
Electrochemistry
electroporation
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Microbiology
Miscellaneous
Plasmids
transformation
Transformation, Bacterial
Vibrio - genetics
Vibrio alginolyticus
Vibrio cholerae
Vibrio cholerae - genetics
Vibrio parahaemolyticus
Vibrio parahaemolyticus - genetics
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Summary:An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1990.tb01047.x