Serological and molecular characterization of three enteric isolates of infectious bronchitis virus of chickens

Three coronaviruses isolated from the intestines of laying chickens were partially characterized. Serological and molecular assays indicated that the enteric coronaviruses are infectious bronchitis virus (IBV) isolates. Although the three isolates were recovered from three unrelated chicken flocks,...

Full description

Saved in:
Bibliographic Details
Published in:Avian diseases 1990-10, Vol.34 (4), p.899-904
Main Authors: Karaca, K. (Cornell University, Ithaca, NY.), Naqi, S.A, Palukaitis, P, Lucio, B
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, Viral - analysis
ARN
BIOCHIMIE
BIOQUIMICA
Chick Embryo
Chickens
Complementary DNA
Coronaviridae Infections - microbiology
Coronaviridae Infections - veterinary
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DNA Probes
ELISA
Embryos
Enzyme linked immunosorbent assay
Genomes
HIBRIDACION DE ADN
HYBRIDATION D'ADN
Immune Sera - immunology
IMMUNOLOGIE
Infectious bronchitis virus
Infectious bronchitis virus - genetics
Infectious bronchitis virus - immunology
INMUNOLOGIA
INTESTIN
Intestines - microbiology
INTESTINOS
METHODE
METODOS
MUTACION
MUTATION
Neutralization Tests
Nucleic Acid Hybridization
Nucleotide Mapping
Oligonucleotides
Oligonucleotides - analysis
POLIMORFISMO GENETICO
POLLO
POLYMORPHISME GENETIQUE
POULET
Poultry Diseases - microbiology
REACCION ANTIGENO-ANTICUERPO
REACTION ANTIGENE ANTICORPS
RNA
RNA, Viral - analysis
SEROTIPOS
SEROTYPE
TEST ELISA
VIRUS BRONCHITE INFECTIEUSE AVIAIRE
VIRUS BRONQUITIS INFECCIOSA AVIAR
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Three coronaviruses isolated from the intestines of laying chickens were partially characterized. Serological and molecular assays indicated that the enteric coronaviruses are infectious bronchitis virus (IBV) isolates. Although the three isolates were recovered from three unrelated chicken flocks, their RNase T1-resistant oligonucleotide fingerprints were almost identical. The three isolates fingerprints were not neutralized by antisera specific to IBV serotype Connecticut, but their RNase T1-resistant oligonucleotide fingerprints closely matched the fingerprints of strain Conn-46, a Connecticut serotype. This and the co-fingerprinting data suggested that the three field isolates may have emerged from the Connecticut virus through mutation(s). The mutation(s) apparently involved the S1 protein gene that determines the virus serotype
ISSN:0005-2086
1938-4351
DOI:10.2307/1591380