A simple method of affinity chromatography for the purification of glucoamylase obtained from Aspergillus niger C

The purpose of this study is to describe a very simple affinity chromatography technique of glucoamylase (EC 3.2.1.3) purification from a filtrate of A. niger C culture. The results suggest an oligomeric structure of this enzyme, a glycoprotein of M sub(1) 50,000-100,000.

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Bibliographic Details
Published in:FEBS letters 1982-11, Vol.149 (1), p.63-66
Main Authors: Paszczyński, Andrzej, Miedziak, Irena, Łobarzewski, Jerzy, Kochmańska, Janina, Trojanowski, Jerzy
Format: Article
Language:English
Subjects:
Affinity chromatography
Aspergillus niger
Aspergillus niger - enzymology
Aspergillus niger C
Characterization
Chromatography, Affinity
exo-1,4- alpha -D-glucosidase
Exosplitting enzyme
Glucan 1,4-alpha-Glucosidase - isolation & purification
Glucan 1,4-alpha-Glucosidase - metabolism
Glucoamylase purification
Glucosidases - isolation & purification
Molecular Weight
purification
Purification, large scale
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Summary:The purpose of this study is to describe a very simple affinity chromatography technique of glucoamylase (EC 3.2.1.3) purification from a filtrate of A. niger C culture. The results suggest an oligomeric structure of this enzyme, a glycoprotein of M sub(1) 50,000-100,000.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)81072-7