A sharp structural transition in pA03 plasmid DNA caused by increased superhelix density

The authors use the 1683 basepair-long plasmid pA03, which is a quarter of the ColE1 plasmid, and contains the main palindromw of ColE1 (26 basepair) The excellent resolution of all topoisomers of pA03 DNA in gel electrophoresis following made it possible to observe a sharp structural transition in...

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Bibliographic Details
Published in:FEBS letters 1982-11, Vol.148 (2), p.297-301
Main Authors: Panyutin, I.G., Lyamichev, V.I., Lyubchenko, Yu.L.
Format: Article
Language:English
Subjects:
Base Sequence
Cruciform structure
DNA Restriction Enzymes
DNA, open/closed circular
DNA, Superhelical - genetics
Electrophoresis, Polyacrylamide Gel
Endonucleases
Escherichia coli
Escherichia coli - genetics
Nucleic Acid Conformation
Palindrome, of pA03 DNA
Plasmid pA03
Plasmids
Single-Strand Specific DNA and RNA Endonucleases
Structural transition
Superhelix density
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Summary:The authors use the 1683 basepair-long plasmid pA03, which is a quarter of the ColE1 plasmid, and contains the main palindromw of ColE1 (26 basepair) The excellent resolution of all topoisomers of pA03 DNA in gel electrophoresis following made it possible to observe a sharp structural transition in pA03 ccDNA with an increase in the number of superhelix turns. The transition is shown to be caused by the formation of a cruciform structure in the main palindrome. The structural transition takes place near - sigma = 0.05, which agrees very well with the theoretically predicted value.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)80828-4