Unconventional application of standard light and electron immunocytochemical analysis to aldehyde-fixed, araldite-embedded tissues

A simple procedure for the immunocytochemical analysis of glutaraldehyde/formaldehyde-fixed, Araldite- or Epon-embedded tissues by either light or electron microscopy is presented. Retention of immunoreactive antigen in deplasticized sections was achieved by use of a low concentration of glutaraldeh...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1982-12, Vol.30 (12), p.1301-1306
Main Authors: Hogan, DL, Smith, GH
Format: Article
Language:English
Subjects:
Animals
Epoxy Resins
Female
Fixatives
Formaldehyde
Glutaral
Immunoenzyme Techniques
Mammary Glands, Animal - analysis
Mammary Neoplasms, Experimental - analysis
Mammary Tumor Virus, Mouse - ultrastructure
Mice
Mice, Inbred C3H
Microscopy
Microscopy, Electron
Phthalic Anhydrides
Proteins - analysis
Skin - analysis
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Summary:A simple procedure for the immunocytochemical analysis of glutaraldehyde/formaldehyde-fixed, Araldite- or Epon-embedded tissues by either light or electron microscopy is presented. Retention of immunoreactive antigen in deplasticized sections was achieved by use of a low concentration of glutaraldehyde in the fixative in combination with a seldom-used plastic solvent. This protocol produced good ultrastructural preservation in tissues and large, high-quality, 2-micrometers thick, plastic-free sections. These semithin sections provided a level of structural and antigenic preservation, image resolution, and labeling intensity that surpassed all other conventional sectioning methods used for immunocytochemistry. The capacity to use a single tissue sample in studies designed for light and electron immunocytochemistry, in conjunction with existing autoradiographic and cytochemical techniques, makes this a very desirable method for routine tissue preparation in research and clinical applications.
ISSN:0022-1554
1551-5044
DOI:10.1177/30.12.6296221